Date published: 2026-6-4

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Villin Antibody (12): sc-136119

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Datasheets
  • Villin Antibody (12) is a mouse monoclonal IgG1, cited in 1 publications, provided at 50 µg/0.5 ml
  • raised against amino acids 1-827 representing full length Villin of bovine origin
  • recommended for detection of Villin of human and bovine origin by WB, IP and IF; not recommended for IP
  • See Villin (1D2C3): sc-58897 for Villin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Villin Antibody (12). This work is in progress.
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Villin Antibody (12) is a mouse monoclonal IgG1 antibody that detects Villin in human and bovine samples through western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) applications. Anti-Villin antibody (12) specifically targets Villin, a crucial actin-binding protein that plays a significant role in the structure and function of brush border microvilli in absorptive epithelial cells. Villin (12) monoclonal antibody recognizes a protein essential for maintaining the integrity and organization of microvilli, which are vital for nutrient absorption in the intestines. Villin is regulated by calcium ions, and Villin′s ability to polymerize actin filaments is critical for dynamic remodeling of the cytoskeleton in response to cellular signals. Villin (12) antibody identifies a protein that contributes to mechanical stability of the brush border and influences cell signaling pathways important for cellular processes such as migration and proliferation. With unique properties and functions, Villin serves as a key player in maintaining cellular architecture and facilitating efficient nutrient uptake, making anti-Villin antibody (12) an invaluable tool for researchers studying epithelial cell biology and related disorders.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Villin Antibody (12)

sc-136119
50 µg/0.5 ml
$322.00

What is the recommended fixation method for immunofluorescence staining with Villin (12): sc-136119 monoclonal antibody?

Asked by: TinTin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Clean band seen in WB using HCTClean band seen in WB using HCT-8 whole cell lysate. -SCBT QC
Date published: 2015-06-13
Rated 4 out of 5 by from Produced wonderful immunofluorescence cytoplasmicProduced wonderful immunofluorescence cytoplasmic staining in HCT-8 cells. -SCBT QC
Date published: 2015-03-05
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Villin Antibody (12) is rated 4.0 out of 5 by 2.
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