Vigilin Double Nickase Plasmid (h): sc-404125-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Vigilin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Vigilin Double Nickase Plasmid (h) and Vigilin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HDLBP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Vigilin Antibody (H-3): sc-271523
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Vigilin Double Nickase Plasmid (h) | sc-404125-NIC | 20 µg | $410.00 | |||
Vigilin Double Nickase Plasmid (h2) | sc-404125-NIC-2 | 20 µg | $410.00 |
Human HDLBP encodes Vigilin, a multifunctional RNA-binding protein containing multiple KH domains that associates with mRNP complexes to regulate mRNA stability, subcellular localization, and translation. Vigilin participates in post-transcriptional control programs linked to lipid and lipoprotein homeostasis, including regulation of transcripts involved in cholesterol handling and metabolic stress responses. Through its coupling of RNA metabolism to cellular growth and adaptation pathways, altered HDLBP/Vigilin activity has been examined in contexts such as dysregulated metabolism and proliferative disease models where RNA regulatory networks are perturbed. These properties make Vigilin a useful target for dissecting RNA-centric mechanisms that shape gene expression outputs in human cells.
Vigilin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HDLBP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HDLBP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HDLBP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HDLBP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.