VHY Antibody (G-7): sc-166173
- VHY Antibody (G-7) is a mouse monoclonal IgG1 κ VHY antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 32-61 near the N-terminus of VHY of human origin
- VHY Antibody (G-7) is recommended for detection of VHY of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine, porcine and avian
- Anti-VHY Antibody (G-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for VHY Antibody (G-7) for WB applications. These reagents are now offered in bundles with VHY Antibody (G-7) (see ordering information below).
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SEE ALSO...
VHY Antibody (G-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects VHY protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). VHY (G-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. VHY protein plays a crucial role in cellular signaling as a member of the dual specificity phosphatases (DSPs), which are essential for regulating the activity of mitogen-activated protein (MAP) kinases. These kinases are pivotal in mediating various physiological responses and cellular adaptations to external stimuli. VHY specifically dephosphorylates critical phosphothreonine and phosphotyrosine residues within MAP kinases, thereby modulating their activity and influencing cellular processes such as growth, differentiation, and stress responses. VHY is highly expressed in the testis, particularly in pachytene spermatocytes and round spermatids, where VHY localizes to the plasma membrane, indicating potential involvement in spermatogenesis and cellular signaling during meiosis. MAP kinase regulation by VHY is vital for maintaining cellular homeostasis and responding to environmental changes, making VHY an important target for research in developmental biology and cancer studies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
VHY Antibody (G-7) References:
- Dual specificity phosphatases: a gene family for control of MAP kinase function. | Camps, M., et al. 2000. FASEB J. 14: 6-16. PMID: 10627275
- VHY, a novel myristoylated testis-restricted dual specificity protein phosphatase related to VHX. | Alonso, A., et al. 2004. J Biol Chem. 279: 32586-91. PMID: 15138252
- The minimal essential core of a cysteine-based protein-tyrosine phosphatase revealed by a novel 16-kDa VH1-like phosphatase, VHZ. | Alonso, A., et al. 2004. J Biol Chem. 279: 35768-74. PMID: 15201283
- Crystal structure of the catalytic domain of human VHY, a dual-specificity protein phosphatase. | Yoon, TS., et al. 2005. Proteins. 61: 694-7. PMID: 16170801
- An emerging family of dual specificity MAP kinase phosphatases. | Keyse, SM. 1995. Biochim Biophys Acta. 1265: 152-60. PMID: 7696343
- Functional studies of dual-specificity phosphatases. | Sun, H. 1998. Methods Mol Biol. 84: 307-18. PMID: 9666459
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VHY Antibody (G-7) | sc-166173 | 200 µg/ml | $316.00 | |||
VHY Antibody (G-7): m-IgG Fc BP-HRP Bundle | sc-527143 | 200 µg Ab; 10 µg BP | $354.00 | |||
VHY Antibody (G-7): m-IgG1 BP-HRP Bundle | sc-532516 | 200 µg Ab; 20 µg BP | $354.00 | |||
VHY Antibody (G-7) AC | sc-166173 AC | 500 µg/ml, 25% agarose | $416.00 | |||
VHY Antibody (G-7) HRP | sc-166173 HRP | 200 µg/ml | $316.00 | |||
VHY Antibody (G-7) FITC | sc-166173 FITC | 200 µg/ml | $330.00 | |||
VHY Antibody (G-7) PE | sc-166173 PE | 200 µg/ml | $343.00 | |||
VHY Antibody (G-7) Alexa Fluor® 488 | sc-166173 AF488 | 200 µg/ml | $357.00 | |||
VHY Antibody (G-7) Alexa Fluor® 546 | sc-166173 AF546 | 200 µg/ml | $357.00 | |||
VHY Antibody (G-7) Alexa Fluor® 594 | sc-166173 AF594 | 200 µg/ml | $357.00 | |||
VHY Antibody (G-7) Alexa Fluor® 647 | sc-166173 AF647 | 200 µg/ml | $357.00 | |||
VHY Antibody (G-7) Alexa Fluor® 680 | sc-166173 AF680 | 200 µg/ml | $357.00 | |||
VHY Antibody (G-7) Alexa Fluor® 790 | sc-166173 AF790 | 200 µg/ml | $357.00 | |||
VHY (G-7) Neutralizing Peptide | sc-166173 P | 100 µg/0.5 ml | $68.00 |