VHR Double Nickase Plasmid (h): sc-405733-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VHR Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- VHR Double Nickase Plasmid (h) and VHR Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DUSP3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: VHR Antibody (F-2): sc-374161
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VHR Double Nickase Plasmid (h) | sc-405733-NIC | 20 µg | $410.00 | |||
VHR Double Nickase Plasmid (h2) | sc-405733-NIC-2 | 20 µg | $410.00 |
Dual specificity phosphatase 3 (DUSP3), also known as vaccinia H1-related phosphatase (VHR), is a cytosolic dual-specificity phosphatase that dephosphorylates phosphotyrosine and phosphoserine/threonine residues on MAPKs, with reported activity toward ERK and JNK family members. By modulating MAPK signaling amplitude and duration, VHR contributes to control of cell-cycle progression, stress-response signaling, and transcriptional programs downstream of growth factor and cytokine inputs. DUSP3 has also been linked to regulation of immune signaling and cellular proliferation through its effects on kinase phosphorylation states and downstream gene expression. Altered DUSP3/VHR expression or activity has been described in contexts of oncogenic signaling and hematologic or solid tumor biology, motivating mechanistic studies of pathway rewiring in disease-relevant models.
VHR Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DUSP3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DUSP3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DUSP3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DUSP3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.