Von Hippel Lindau/VHL Antibody (D-7): sc-55506

VHL Antibody (D-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects VHL in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-VHL antibody (D-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The von Hippel-Lindau (VHL) protein plays a crucial role in regulating cellular responses to hypoxia by targeting hypoxia-inducible factors (HIFs) for degradation, thereby controlling gene expression involved in angiogenesis and erythropoiesis. This function maintains cellular homeostasis and prevents tumorigenesis, as dysregulation of HIFs can lead to excessive blood vessel formation and contribute to various cancers. Individuals with germline mutations in the VHL gene are predisposed to several tumor types, including renal cell carcinoma, hemangioblastoma, and pheochromocytoma, highlighting VHL′s importance in tumor suppression. The Elongin (SIII) complex, including Elongin A, B, and C, is a key functional target of VHL, as VHL binds to regulatory subunits to inhibit transcriptional activity. Different VHL isoforms, resulting from alternatively spliced transcript variants, exhibit varying molecular weights across species, emphasizing this tumor suppressor′s complexity and significance in cancer biology.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Von Hippel Lindau/VHL Antibody (D-7) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Von Hippel Lindau/VHL Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Von Hippel Lindau/VHL Antibody (D-7) Specifications

Alternate Product Name: von Hippel-Lindau tumor suppressor

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-181

Epitope Target: VHL (von Hippel-Lindau tumor suppressor protein)

Epitope Species: mouse

Product Reactivity/Detection: VHL

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Von Hippel Lindau/VHL Antibody (D-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Von Hippel Lindau/VHL antibody is Von Hippel Lindau/VHL Antibody (VHL40): sc-135657.