VGAT Antibody (F-2): sc-393373

VGAT Antibody (F-2) is a mouse monoclonal IgM kappa antibody that detects VGAT protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-VGAT antibody (F-2) is available as the non-conjugated form. The vesicular GABA transporter (VGAT) plays a crucial role in synaptic transmission by facilitating the loading of gamma-aminobutyric acid (GABA), an essential inhibitory neurotransmitter, into synaptic vesicles. This process is vital for maintaining the balance of excitatory and inhibitory signals in the nervous system, which is critical for proper neuronal function and overall brain health. VGAT is predominantly expressed in the nerve endings of inhibitory neurons that contain GABA and/or glycine, and VGAT expression is tightly regulated during neocortical development, where VGAT precedes the maturation of inhibitory synaptogenesis. This suggests that VGAT is not only important for neurotransmitter loading but may also contribute to the formation of GABAergic circuitry in the developing brain. Furthermore, alterations in VGAT function have been implicated in various neurological conditions, including epilepsy, highlighting VGAT significance in both normal physiology and disease states.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

VGAT Antibody (F-2) References:

1. Postnatal development of the vesicular GABA transporter in rat cerebral cortex.  |  Minelli, A., et al. 2003. Neuroscience. 117: 337-46. PMID: 12614674
2. Ethanol induces GAD67 and VGAT in slice cultures of newborn rat cerebral cortex.  |  Zink, M. and Spanagel, R. 2005. Neuroreport. 16: 377-80. PMID: 15729141
3. The mouse vesicular inhibitory amino acid transporter gene: expression during embryogenesis, analysis of its core promoter in neural stem cells and a reconsideration of its alternate splicing.  |  Oh, WJ., et al. 2005. Gene. 351: 39-49. PMID: 15826867
4. Decreased expression of vesicular GABA transporter, but not vesicular glutamate, acetylcholine and monoamine transporters in rat brain following focal ischemia.  |  Vemuganti, R. 2005. Neurochem Int. 47: 136-42. PMID: 15936116
5. Remodeling of hippocampal GABAergic system in adult offspring after maternal hypoxia and magnesium sulfate load: immunohistochemical study.  |  Gerstein, M., et al. 2005. Exp Neurol. 196: 18-29. PMID: 16081066
6. Heterogeneity of axon terminals expressing VGLUT1 in the cerebral neocortex.  |  Conti, F., et al. 2005. Arch Ital Biol. 143: 127-32. PMID: 16106993
7. Receptors and sites of synthesis and storage of gamma-aminobutyric acid in human pituitary glands and in growth hormone adenomas.  |  End, K., et al. 2005. Am J Clin Pathol. 124: 550-8. PMID: 16146821
8. Absence of synapsin I and II is accompanied by decreases in vesicular transport of specific neurotransmitters.  |  Bogen, IL., et al. 2006. J Neurochem. 96: 1458-66. PMID: 16478532
9. Stable expression of the vesicular GABA transporter following photothrombotic infarct in rat brain.  |  Frahm, C., et al. 2006. Neuroscience. 140: 865-77. PMID: 16616431
10. Impaired glycinergic synaptic transmission and enhanced inflammatory pain in mice with reduced expression of vesicular GABA transporter (VGAT).  |  Yamada, MH., et al. 2012. Mol Pharmacol. 81: 610-9. PMID: 22275517
11. Activation of ventral tegmental area vesicular GABA transporter (Vgat) neurons alleviates social defeat stress-induced anxiety in APP/PS1 mice.  |  Yao, D., et al. 2023. Front Aging Neurosci. 15: 1142055. PMID: 37032820
12. Expression of the vesicular GABA transporter within neuromedin S+ neurons sustains behavioral circadian rhythms.  |  Bussi, IL., et al. 2023. Proc Natl Acad Sci U S A. 120: e2314857120. PMID: 38019855

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-VGAT Antibody (F-2) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-VGAT Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

VGAT Antibody (F-2) Specifications

Alternate Product Name: VGAT

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 71-98

Epitope Target: VGAT

Epitope Species: human

Product Reactivity/Detection: VGAT

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for VGAT Antibody (F-2): P: Blocking Peptide