Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
VDUP1 Double Nickase Plasmid (h): sc-400664-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VDUP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- VDUP1 Double Nickase Plasmid (h) and VDUP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TXNIP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: VDUP1 Antibody (D-2): sc-271237
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VDUP1 Double Nickase Plasmid (h) | sc-400664-NIC | 20 µg | $410.00 | |||
VDUP1 Double Nickase Plasmid (h2) | sc-400664-NIC-2 | 20 µg | $410.00 |
TXNIP, also known as VDUP1, encodes a thioredoxin-interacting protein that constrains cellular redox buffering by inhibiting thioredoxin activity, thereby linking oxidative stress to downstream signaling outcomes. It is induced by metabolic and endoplasmic reticulum stress and integrates inputs from glucose sensing, inflammatory cues, and transcriptional regulators to modulate apoptosis, proliferation, and cellular metabolism. TXNIP participates in pathways governing reactive oxygen species homeostasis and can promote NLRP3 inflammasome activation in contexts where oxidative and metabolic stress converge. Dysregulated TXNIP expression has been associated with cardiometabolic disorders, neuroinflammation, and tumor biology, making it a useful node for mechanistic studies of stress adaptation and immunometabolic signaling.
VDUP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TXNIP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TXNIP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TXNIP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TXNIP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.