Vitamin D Receptor/VDR (m): 293T Lysate: sc-124548

VDR (m): 293T Lysate is derived from the human embryonic kidney 293T cell line, genetically engineered to express the mouse Vitamin D Receptor (VDR). VDR is a nuclear receptor that mediates the effects of vitamin D by regulating gene expression involved in calcium homeostasis, immune response, and cellular growth. The 293T cell line, chosen for its high transfection efficiency and robust protein production, is ideal for producing VDR lysates. In research, VDR (m): 293T Lysate is used to investigate the molecular mechanisms of vitamin D signaling. VDR functions by binding to the active form of vitamin D, 1,25-dihydroxyvitamin D3, which induces conformational changes that allow VDR to heterodimerize with the retinoid X receptor (RXR). This complex then binds to vitamin D response elements (VDREs) in the promoter regions of target genes, modulating their transcription. Researchers use this lysate in various biochemical assays, including Western blotting, immunoprecipitation, and electrophoretic mobility shift assays (EMSAs), to analyze VDR expression, DNA-binding activity, and interactions with co-regulators. Additionally, VDR (m): 293T Lysate is valuable in chromatin immunoprecipitation (ChIP) assays to study VDR's role in chromatin remodeling and gene regulation. This lysate helps explain the signaling pathways that modulate VDR activity and its cross-talk with other signaling networks. By using VDR (m): 293T Lysate, researchers gain insights into the regulatory mechanisms of VDR, enhancing our understanding of vitamin D's role in various biological processes and its broader impact on cellular function.