VCX-C CRISPR/Cas9 KO Plasmid (h): sc-416596
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VCX-C CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the VCX-C genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VCX-C CRISPR/Cas9 KO Plasmid (h) | sc-416596 | 20 µg | $397.00 |
Overview
VCX3B encodes VCX-C, a member of the VCX/Y protein family enriched in male germline tissues and associated with testis-specific transcriptional programs. Proteins in this family are implicated in regulating mRNA handling and post-transcriptional control during spermatogenesis, linking VCX-C to processes such as RNA metabolism and germ cell differentiation. VCX3B resides in the X chromosome region that is recurrently involved in copy-number variation, making it relevant for studies of dosage sensitivity and genome stability. Dysregulation of VCX family genes has been explored in the context of male infertility phenotypes and X-linked genomic rearrangements, supporting mechanistic investigations in reproductive and developmental biology models.
VCX-C CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the VCX3B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the VCX3B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the VCX3B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish VCX-C protein expression.
This CRISPR knockout system enables efficient generation of VCX3B-deficient cell models for investigation of VCX-C signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting VCX3B exon(s) critical for VCX-C function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple VCX3B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by VCX-C CRISPR/Cas9 KO Plasmid (h) and VCX-C CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the VCX3B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by VCX-C HDR Plasmid (h) and VCX-C HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by VCX3B homology arms to support homology-directed repair at defined VCX3B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.