Vav3 Antibody (F-11): sc-518196

Vav3 Antibody (F-11) is a mouse monoclonal IgM antibody that detects Vav3 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Vav3 Antibody (F-11) is available in a non-conjugated form, providing flexibility for various experimental needs. Vav3 is a member of the Vav family of Rho guanine nucleotide exchange factors (GEFs), which play a crucial role in orchestrating signaling events following lymphocyte antigen receptor activation. One of the key aspects of Vav3 is its interaction with specific adaptor proteins, such as SLP76 and 3BP2, which are essential for downstream signaling pathways. Notably, Vav3 undergoes tyrosine phosphorylation in response to T cell receptor cross-linkage, leading to activation and subsequent engagement with these adaptor molecules. This interaction is vital for the regulation of gene transcription linked to serum response elements, distinguishing Vav3′s function from that of Vav1, which primarily influences interleukin-2 promoter activity. Additionally, Vav3 exhibits a cell cycle-dependent expression pattern, with transient upregulation during mitosis, suggesting involvement in cytokinesis. The ability of enforced Vav3 expression to induce multinucleate cell formation further underscores a significant role in cell division and maintenance of cellular integrity.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Vav3 Antibody (F-11) References:

1. Interferon-alpha signaling promotes nucleus-to-cytoplasmic redistribution of p95Vav, and formation of a multisubunit complex involving Vav, Ku80, and Tyk2.  |  Adam, L., et al. 2000. Biochem Biophys Res Commun. 267: 692-6. PMID: 10673353
2. Vav3 is regulated during the cell cycle and effects cell division.  |  Fujikawa, K., et al. 2002. Proc Natl Acad Sci U S A. 99: 4313-8. PMID: 11917103
3. Vav1/2/3-null mice define an essential role for Vav family proteins in lymphocyte development and activation but a differential requirement in MAPK signaling in T and B cells.  |  Fujikawa, K., et al. 2003. J Exp Med. 198: 1595-608. PMID: 14623913
4. Differential regulation of TCR-mediated gene transcription by Vav family members.  |  Zakaria, S., et al. 2004. J Exp Med. 199: 429-34. PMID: 14757747
5. Vav3 oncogene is overexpressed and regulates cell growth and androgen receptor activity in human prostate cancer.  |  Dong, Z., et al. 2006. Mol Endocrinol. 20: 2315-25. PMID: 16762975
6. Vav3 proto-oncogene deficiency leads to sympathetic hyperactivity and cardiovascular dysfunction.  |  Sauzeau, V., et al. 2006. Nat Med. 12: 841-5. PMID: 16767097
7. Activation of Rac1 and the exchange factor Vav3 are involved in NPM-ALK signaling in anaplastic large cell lymphomas.  |  Colomba, A., et al. 2008. Oncogene. 27: 2728-36. PMID: 17998938
8. Nuclear Vav3 is required for polycomb repression complex-1 activity in B-cell lymphoblastic leukemogenesis.  |  Nayak, RC., et al. 2022. Nat Commun. 13: 3056. PMID: 35650206
9. VAV3 in human cancers: Mechanism and clinical implication.  |  Al-Hawary, SIS., et al. 2023. Pathol Res Pract. 248: 154681. PMID: 37467637
10. The role of STAT3/VAV3 in glucolipid metabolism during the development of HFD-induced MAFLD.  |  Jiang, Y., et al. 2024. Int J Biol Sci. 20: 2027-2043. PMID: 38617550
11. DDX5 promotes esophageal squamous cell carcinoma growth through sustaining VAV3 mRNA stability.  |  Shi, Y., et al. 2024. Oncogene. 43: 3240-3254. PMID: 39289531
12. p95vav associates with the nuclear protein Ku-70.  |  Romero, F., et al. 1996. Mol Cell Biol. 16: 37-44. PMID: 8524317

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Vav3 Antibody (F-11) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Vav3 Antibody (F-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Vav3 Antibody (F-11) Specifications

Alternate Product Name: Vav3

Product Clone Name: F-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 122-146

Epitope Target: Vav3

Epitope Species: human

Product Reactivity/Detection: Vav3

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Vav3 Antibody (F-11): N/A