VASP (h): 293T Lysate: sc-114829

VASP (h): 293T Lysate is derived from the human embryonic kidney 293T cell line, genetically engineered to express human Vasodilator-Stimulated Phosphoprotein (VASP). VASP is a member of the Ena/VASP family of proteins that play a crucial role in the regulation of actin cytoskeleton dynamics, which are essential for cell motility, adhesion, and signal transduction. The 293T cell line, renowned for its high transfection efficiency and robust protein expression, is an ideal system for producing VASP lysates. In research, VASP (h): 293T Lysate is utilized to investigate the molecular mechanisms of actin polymerization and cell motility. VASP functions by promoting actin filament elongation and protecting actin filaments from capping proteins, thus facilitating cell movement and adhesion. Researchers employ this lysate in various biochemical assays, such as Western blotting, immunoprecipitation, and immunofluorescence, to analyze VASP expression, post-translational modifications, and interactions with other cytoskeletal proteins. Additionally, VASP (h): 293T Lysate is used in functional assays to study the effects of VASP on cell migration and adhesion. This lysate helps to explain the signaling pathways that regulate VASP activity, such as those involving protein kinases like PKA and PKG, which phosphorylate VASP and modulate its function. By using VASP (h): 293T Lysate, researchers gain insights into the dynamic processes governing cell movement and the role of VASP in cellular functions. This research advances our understanding of cytoskeletal dynamics and cell biology.