VAChT Antibody (H-10): sc-518260
- VAChT Antibody (H-10) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 484-509 of VAChT of human origin
- recommended for detection of VAChT of human origin by WB, IP, IF and ELISA
- Anti-VAChT Antibody (H-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for VAChT Antibody (H-10) for WB applications. This reagent is now offered in a bundle with VAChT Antibody (H-10) (see ordering information below).
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VAChT Antibody (H-10) is an IgG1 κ mouse monoclonal VAChT antibody (also designated VAChT antibody) that detects VAChT of human origin by WB, IP, IF and ELISA. VAChT Antibody (H-10) is available as the non-conjugated anti-VAChT antibody form, as well as multiple conjugated forms of anti-VAChT antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Neurotransmission depends on the regulated exocytotic release of chemical transmitter molecules. This requires the packaging of these substances into the specialized secretory vesicles of neurons and neuroendocrine cells, a process mediated by specific vesicular transporters. The family of genes encoding the vesicular transporters of monoamines (VMAT 1 and VMAT 2) and acetylcholine (VACht) have been cloned and functionally characterized. The sequence of these integral membrane proteins predicts twelve transmembrane domains and weak homology to a class of bacterial antibiotic resistance proteins. The vesicular transport of neurotransmitter molecules has been shown to be an active ATP- and proton dependent transport mechanism.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
VAChT Antibody (H-10) References:
- Mice deficient for striatal Vesicular Acetylcholine Transporter (VAChT) display impaired short-term but normal long-term object recognition memory. | Palmer, D., et al. 2016. Behav Brain Res. 311: 267-278. PMID: 27233822
- Variants in SLC18A3, vesicular acetylcholine transporter, cause congenital myasthenic syndrome. | O'Grady, GL., et al. 2016. Neurology. 87: 1442-1448. PMID: 27590285
- Vesicular acetylcholine transporter (VAChT) over-expression induces major modifications of striatal cholinergic interneuron morphology and function. | Janickova, H., et al. 2017. J Neurochem. 142: 857-875. PMID: 28628197
- Biochemistry and molecular biology of the vesicular monoamine transporter from chromaffin granules. | Henry, JP., et al. 1994. J Exp Biol. 196: 251-62. PMID: 7823026
- Distribution of the vesicular acetylcholine transporter (VAChT) in the central and peripheral nervous systems of the rat. | Schäfer, MK., et al. 1994. J Mol Neurosci. 5: 1-26. PMID: 7857778
- Molecular cloning of a putative vesicular transporter for acetylcholine. | Roghani, A., et al. 1994. Proc Natl Acad Sci U S A. 91: 10620-4. PMID: 7938002
- Acetylcholine active transport by rat brain synaptic vesicles. | Haigh, JR., et al. 1994. Neuroreport. 5: 773-6. PMID: 8018848
- The pharmacological profile of the vesicular monoamine transporter resembles that of multidrug transporters. | Yelin, R. and Schuldiner, S. 1995. FEBS Lett. 377: 201-7. PMID: 8543051
- Active transport of acetylcholine by the human vesicular acetylcholine transporter. | Varoqui, H. and Erickson, JD. 1996. J Biol Chem. 271: 27229-32. PMID: 8910293
- Vesicular acetylcholine transporter (VAChT): a cellular marker in rat retinal development. | Koulen, P. 1997. Neuroreport. 8: 2845-8. PMID: 9376516
- Vesicular neurotransmitter transporters. Potential sites for the regulation of synaptic function. | Varoqui, H. and Erickson, JD. 1997. Mol Neurobiol. 15: 165-91. PMID: 9396009
- Vesicular neurotransmitter transport and the presynaptic regulation of quantal size. | Reimer, RJ., et al. 1998. Curr Opin Neurobiol. 8: 405-12. PMID: 9687352
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VAChT Antibody (H-10) | sc-518260 | 200 µg/ml | $322.00 | |||
VAChT Antibody (H-10): m-IgG1 BP-HRP Bundle | sc-551762 | 200 µg Ab; 20 µg BP | $361.00 | |||
VAChT Antibody (H-10) AC | sc-518260 AC | 500 µg/ml, 25% agarose | $424.00 | |||
VAChT Antibody (H-10) HRP | sc-518260 HRP | 200 µg/ml | $322.00 | |||
VAChT Antibody (H-10) FITC | sc-518260 FITC | 200 µg/ml | $336.00 | |||
VAChT Antibody (H-10) PE | sc-518260 PE | 200 µg/ml | $349.00 | |||
VAChT Antibody (H-10) Alexa Fluor® 488 | sc-518260 AF488 | 200 µg/ml | $364.00 | |||
VAChT Antibody (H-10) Alexa Fluor® 546 | sc-518260 AF546 | 200 µg/ml | $364.00 | |||
VAChT Antibody (H-10) Alexa Fluor® 594 | sc-518260 AF594 | 200 µg/ml | $364.00 | |||
VAChT Antibody (H-10) Alexa Fluor® 647 | sc-518260 AF647 | 200 µg/ml | $364.00 | |||
VAChT Antibody (H-10) Alexa Fluor® 680 | sc-518260 AF680 | 200 µg/ml | $364.00 | |||
VAChT Antibody (H-10) Alexa Fluor® 790 | sc-518260 AF790 | 200 µg/ml | $364.00 |