VacA Antibody (5E4): sc-32746
- VacA Antibody (5E4) is a mouse monoclonal IgG1 κ VacA antibody, cited in 6 publications, provided at 200 µg/ml
- raised against amino acids 685-821 of purified native s1-m1 VacA
- VacA Antibody (5E4) is recommended for detection of VacA of H. pylori origin by IP and IF; non cross-reactive with type s2-m2 VacA or denatured type s1-m1 VacA
- Anti-VacA Antibody (5E4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also provided azide-free for VacA toxin neutralizing activity, sc-32746 L, 200 µg/0.1 ml
- m-IgG1 BP-HRP is the preferred secondary detection reagent for VacA Antibody (5E4). This reagent is now offered in a bundle with VacA Antibody (5E4) (see ordering information below).
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VacA Antibody (5E4) is a mouse monoclonal IgG1 kappa light chain antibody that detects the VacA protein of H. pylori origin by immunoprecipitation (IP) and immunofluorescence (IF). VacA (5E4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. VacA protein plays a crucial role in the pathogenesis of Helicobacter pylori, a spiral-shaped bacterium responsible for most stomach and duodenal ulcers, as well as being linked to gastric cancer development. VacA functions by altering the endocytic pathway in non-polarized cells, leading to the release of acid hydrolases and diminishing the degradation of extracellular ligands and antigen processing. This mechanism allows the bacterium to evade the host′s immune response, contributing to chronic infection. VacA forms transmembrane anion-specific channels that reduce the transepithelial electrical resistance of polarized monolayers, causing osmotic swelling and subsequent vacuole formation in acidic intracellular compartments. VacA serves as a key antigen in the human immune response to H. pylori infection. VacA interacts with cytotoxin-associated gene A (CagA), which induces morphological changes in host cells, including actin reorganization and alterations in the cell cycle, thereby enhancing the pathogenicity of H. pylori.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
VacA Antibody (5E4) References:
- Mechanisms of Helicobacter pylori infection: bacterial factors. | McGee, DJ. and Mobley, HL. 1999. Curr Top Microbiol Immunol. 241: 155-80. PMID: 10087661
- Helicobacter pylori associated gastric pathology. | Konturek, PC., et al. 1999. J Physiol Pharmacol. 50: 695-710. PMID: 10695552
- Disease-specific Helicobacter pylori virulence factors: the unfulfilled promise. | Graham, DY. and Yamaoka, Y. 2000. Helicobacter. 5 Suppl 1: S3-9; discussion S27-31. PMID: 10828748
- Cellular responses induced after contact with Helicobacter pylori. | Censini, S., et al. 2001. Curr Opin Microbiol. 4: 41-6. PMID: 11173032
- Virulence factors of Helicobacter pylori. | Dundon, WG., et al. 2001. Int J Med Microbiol. 290: 647-58. PMID: 11310443
- Increased risk of developing atrophic gastritis in patients infected with CagA+ Helicobacter pylori. | Sande, N., et al. 2001. Scand J Gastroenterol. 36: 928-33. PMID: 11521982
- Vacuolating cytotoxin A (VacA) - A multi-talented pore-forming toxin from Helicobacter pylori. | Junaid, M., et al. 2016. Toxicon. 118: 27-35. PMID: 27105670
- New Insights into VacA Intoxication Mediated through Its Cell Surface Receptors. | Yahiro, K., et al. 2016. Toxins (Basel). 8: PMID: 27187473
- An Overview of Helicobacter pylori VacA Toxin Biology. | Foegeding, NJ., et al. 2016. Toxins (Basel). 8: PMID: 27271669
- Pleiotropic cytotoxicity of VacA toxin in host cells and its impact on immunotherapy. | Fahimi, F., et al. 2017. Bioimpacts. 7: 59-71. PMID: 28546954
- Structural Analysis of Membrane-associated Forms of Helicobacter pylori VacA Toxin. | Connolly, SM., et al. 2024. J Mol Biol. 436: 168432. PMID: 38161000
- Taurine modulates host cell responses to Helicobacter pylori VacA toxin. | Westland, MD., et al. 2024. Infect Immun. 92: e0022424. PMID: 38975764
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VacA Antibody (5E4) | sc-32746 | 200 µg/ml | $316.00 | |||
VacA Antibody (5E4): m-IgG1 BP-HRP Bundle | sc-531643 | 200 µg Ab; 20 µg BP | $354.00 | |||
VacA Antibody (5E4) L | sc-32746 L | 200 µg/0.1 ml | $316.00 | |||
VacA Antibody (5E4) AC | sc-32746 AC | 500 µg/ml, 25% agarose | $416.00 | |||
VacA Antibody (5E4) HRP | sc-32746 HRP | 200 µg/ml | $316.00 | |||
VacA Antibody (5E4) FITC | sc-32746 FITC | 200 µg/ml | $330.00 | |||
VacA Antibody (5E4) PE | sc-32746 PE | 200 µg/ml | $343.00 | |||
VacA Antibody (5E4) Alexa Fluor® 488 | sc-32746 AF488 | 200 µg/ml | $357.00 | |||
VacA Antibody (5E4) Alexa Fluor® 546 | sc-32746 AF546 | 200 µg/ml | $357.00 | |||
VacA Antibody (5E4) Alexa Fluor® 594 | sc-32746 AF594 | 200 µg/ml | $357.00 | |||
VacA Antibody (5E4) Alexa Fluor® 647 | sc-32746 AF647 | 200 µg/ml | $357.00 | |||
VacA Antibody (5E4) Alexa Fluor® 680 | sc-32746 AF680 | 200 µg/ml | $357.00 | |||
VacA Antibody (5E4) Alexa Fluor® 790 | sc-32746 AF790 | 200 µg/ml | $357.00 |