V-ATPase D2 Antibody (7A4): sc-517031

V-ATPase D2 Antibody (7A4) is a mouse monoclonal IgG2b kappa light chain antibody that detects V-ATPase D2 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-V-ATPase D2 antibody (7A4) is available as the non-conjugated format. V-ATPase D2, consisting of 350 amino acids, plays a crucial role in the acidification of eukaryotic intracellular organelles, which is essential for various cellular processes, including protein degradation, nutrient absorption, and pH regulation within organelles. V-ATPase D2 is primarily located in the kidney, lung, and osteoclasts, where V-ATPase D2 is involved in urine acidification, osteoclast fusion, and bone formation. The V-ATPase complex is composed of two main domains: the peripheral V1 domain, responsible for ATP hydrolysis, and the integral V0 domain, which facilitates proton translocation. The intricate structure of V-ATPase, with nine subunits in the V1 domain and five subunits in the V0 domain, allows V-ATPase to operate through a rotary mechanism similar to F-ATPases, thereby efficiently pumping protons against an electrochemical gradient. V-ATPase D2 has been identified as a dendritic cell marker, highlighting V-ATPase D2′s importance in immune responses. V-ATPase D2 monoclonal antibody (7A4) is an invaluable tool for researchers studying the functional dynamics of V-ATPase D2 in various biological contexts.

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V-ATPase D2 Antibody (7A4) References:

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6. v-ATPase V0 subunit d2-deficient mice exhibit impaired osteoclast fusion and increased bone formation.  |  Lee, SH., et al. 2006. Nat Med. 12: 1403-9. PMID: 17128270
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