V-ATPase C1 Double Nickase Plasmid (h): sc-402679-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- V-ATPase C1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- V-ATPase C1 Double Nickase Plasmid (h) and V-ATPase C1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ATP6V1C1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: V-ATPase C1 Antibody (G-5): sc-271077
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
V-ATPase C1 Double Nickase Plasmid (h) | sc-402679-NIC | 20 µg | $410.00 | |||
V-ATPase C1 Double Nickase Plasmid (h2) | sc-402679-NIC-2 | 20 µg | $410.00 |
ATP6V1C1 encodes the C1 subunit of the human vacuolar H+-ATPase (V-ATPase) V1 domain, a core component of the cytosolic ATP-hydrolyzing sector that powers proton translocation across endomembrane and plasma membranes. V-ATPase-driven acidification is essential for endosome–lysosome maturation, receptor-mediated endocytosis, autophagic flux, and vesicular trafficking, and it supports pH-dependent processing of macromolecules within the secretory and degradative pathways. Through regulation of organelle pH and coupling to nutrient-sensing networks such as mTORC1 at the lysosome, V-ATPase activity influences cellular metabolism, stress responses, and membrane protein turnover. Dysregulated V-ATPase function and altered compartmental pH homeostasis have been associated with phenotypes relevant to neurodegeneration, tumor cell invasion/metastatic potential, and defects in antigen processing and immune signaling, making ATP6V1C1 a useful target for mechanistic studies of acidification-dependent biology.
V-ATPase C1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ATP6V1C1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ATP6V1C1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ATP6V1C1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ATP6V1C1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.