UVSSA CRISPR/Cas9 KO Plasmid (h): sc-408933
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UVSSA CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UVSSA genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UVSSA CRISPR/Cas9 KO Plasmid (h) | sc-408933 | 20 µg | $397.00 |
Overview
UVSSA (UV-stimulated scaffold protein A) is a key factor in transcription-coupled nucleotide excision repair (TC-NER), where it helps restore transcription after RNA polymerase II stalls at UV-induced DNA lesions. The human UVSSA protein cooperates with CSA/ERCC8 and CSB/ERCC6 and promotes recruitment and stabilization of the USP7 deubiquitinase, supporting regulated ubiquitination dynamics during repair complex assembly. Through these activities, UVSSA contributes to genome integrity, recovery of transcription following genotoxic stress, and cellular responses to UV irradiation. Defects in UVSSA-mediated TC-NER are linked to UV sensitivity and disorders within the Cockayne syndrome spectrum, making it relevant for studying DNA repair pathway dysfunction and stress-induced transcriptional failure.
UVSSA CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the UVSSA gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the UVSSA together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the UVSSA open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UVSSA protein expression.
This CRISPR knockout system enables efficient generation of UVSSA-deficient cell models for investigation of UVSSA signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting UVSSA exon(s) critical for UVSSA function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple UVSSA genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UVSSA CRISPR/Cas9 KO Plasmid (h) and UVSSA CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the UVSSA locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UVSSA HDR Plasmid (h) and UVSSA HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by UVSSA homology arms to support homology-directed repair at defined UVSSA target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.