UTY Antibody (G-3): sc-514690
- UTY Antibody (G-3) is a mouse monoclonal IgG1 κ UTY antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 599-616 within an internal region of UTY of human origin
- UTY Antibody (G-3) is recommended for detection of UTY of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-UTY Antibody (G-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of UTY (G-3): sc-514690.
- m-IgG1 BP-HRP is the preferred secondary detection reagent for UTY Antibody (G-3) for WB applications. This reagent is now offered in a bundle with UTY Antibody (G-3) (see ordering information below).
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UTY Antibody (G-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects UTY protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). UTY (G-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UTY protein, a product of the ubiquitously transcribed tetratricopeptide-repeat genes located on the Y chromosome, plays a crucial role in gene regulation by interacting with members of the Groucho/transducin-like Enhancer of split (TLE) family, which are vertebrate orthologs of the yeast Tup1 protein. This interaction is vital for forming transcriptional repressor complexes that modulate gene expression, thereby influencing various biological processes, including development and differentiation. Additionally, UTY′s functional relationship with TLE1 and TLE2 highlights UTY′s importance in regulating specific gene targets, which can have significant implications in understanding sex-linked genetic disorders and broader mechanisms of gene regulation. A related gene on the X chromosome encodes UTX protein, which shares functional similarities with UTY, further emphasizing the intricate balance of gene expression regulation between sex chromosomes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
UTY Antibody (G-3) References:
- Recruitment of Tup1-Ssn6 by yeast hypoxic genes and chromatin-independent exclusion of TATA binding protein. | Mennella, TA., et al. 2003. Eukaryot Cell. 2: 1288-303. PMID: 14665463
- Modelling transcriptional feedback loops: the role of Gro/TLE1 in Hes1 oscillations. | Bernard, S., et al. 2006. Philos Trans A Math Phys Eng Sci. 364: 1155-70. PMID: 16608701
- Transcriptional repression by Tup1-Ssn6. | Malavé, TM. and Dent, SY. 2006. Biochem Cell Biol. 84: 437-43. PMID: 16936817
- TLE1 as a diagnostic immunohistochemical marker for synovial sarcoma emerging from gene expression profiling studies. | Terry, J., et al. 2007. Am J Surg Pathol. 31: 240-6. PMID: 17255769
- A complex composed of tup1 and ssn6 represses transcription in vitro. | Redd, MJ., et al. 1997. J Biol Chem. 272: 11193-7. PMID: 9111019
- Groucho/transducin-like enhancer of split (TLE) family members interact with the yeast transcriptional co-repressor SSN6 and mammalian SSN6-related proteins: implications for evolutionary conservation of transcription repression mechanisms. | Grbavec, D., et al. 1999. Biochem J. 337 (Pt 1): 13-7. PMID: 9854018
- Transducin-like Enhancer of split 2, a mammalian homologue of Drosophila Groucho, acts as a transcriptional repressor, interacts with Hairy/Enhancer of split proteins, and is expressed during neuronal development. | Grbavec, D., et al. 1998. Eur J Biochem. 258: 339-49. PMID: 9874198
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UTY Antibody (G-3) | sc-514690 | 200 µg/ml | $316.00 | |||
UTY Antibody (G-3): m-IgG1 BP-HRP Bundle | sc-531790 | 200 µg Ab; 20 µg BP | $354.00 | |||
UTY Antibody (G-3) AC | sc-514690 AC | 500 µg/ml, 25% agarose | $416.00 | |||
UTY Antibody (G-3) HRP | sc-514690 HRP | 200 µg/ml | $316.00 | |||
UTY Antibody (G-3) FITC | sc-514690 FITC | 200 µg/ml | $330.00 | |||
UTY Antibody (G-3) PE | sc-514690 PE | 200 µg/ml | $343.00 | |||
UTY Antibody (G-3) Alexa Fluor® 488 | sc-514690 AF488 | 200 µg/ml | $357.00 | |||
UTY Antibody (G-3) Alexa Fluor® 546 | sc-514690 AF546 | 200 µg/ml | $357.00 | |||
UTY Antibody (G-3) Alexa Fluor® 594 | sc-514690 AF594 | 200 µg/ml | $357.00 | |||
UTY Antibody (G-3) Alexa Fluor® 647 | sc-514690 AF647 | 200 µg/ml | $357.00 | |||
UTY Antibody (G-3) Alexa Fluor® 680 | sc-514690 AF680 | 200 µg/ml | $357.00 | |||
UTY Antibody (G-3) Alexa Fluor® 790 | sc-514690 AF790 | 200 µg/ml | $357.00 | |||
UTY (G-3) Neutralizing Peptide | sc-514690 P | 100 µg/0.5 ml | $68.00 |