Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
UTX Double Nickase Plasmid (h): sc-402761-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UTX Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- UTX Double Nickase Plasmid (h) and UTX Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KDM6A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UTX Antibody (E-8): sc-514859
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UTX Double Nickase Plasmid (h) | sc-402761-NIC | 20 µg | $410.00 | |||
UTX Double Nickase Plasmid (h2) | sc-402761-NIC-2 | 20 µg | $410.00 |
KDM6A encodes UTX, an H3K27me3-specific histone demethylase that counterbalances Polycomb-mediated repression to promote transcriptional activation. UTX functions within chromatin remodeling complexes and regulates lineage specification, cell-cycle control, and DNA damage–responsive transcriptional programs through epigenetic remodeling. By shaping enhancer and promoter accessibility, KDM6A influences developmental gene networks and immune-related signaling pathways. Dysregulation or loss of KDM6A is linked to aberrant chromatin states observed across multiple cancer types and congenital developmental disorders, supporting its use as a model for epigenetic disease mechanisms.
UTX Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KDM6A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KDM6A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KDM6A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KDM6A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.