USP9X/Y Antibody (E-12): sc-365353

USP9X/Y Antibody (E-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects USP9X/Y protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). USP9X/Y (E-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. USP9X protein, a member of the peptidase C19 family of ubiquitin proteases, plays a crucial role in the ubiquitin-proteasome pathway, which is essential for regulating protein degradation and turnover within the cell. This pathway is vital for maintaining cellular homeostasis, as USP9X/Y controls various intracellular processes such as signal transduction, transcriptional activation, and cell cycle progression. USP9X is expressed ubiquitously in both fetal and adult tissues, where USP9X processes ubiquitin precursors and ubiquitinated proteins, thereby preventing their degradation. Additionally, USP9Y, the Y-linked counterpart, also contributes to these processes, and both proteins are implicated in critical reproductive functions. Defects in USP9X gene are associated with Turner syndrome, characterized by impaired oocyte development, while abnormalities in USP9Y are linked to non-obstructive azoospermia and infertility. Anti-USP9X/Y antibody (E-12) effectively detects these proteins, making USP9X/Y (E-12) monoclonal antibody an invaluable tool for researchers studying the ubiquitin pathway and its implications in various diseases.

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USP9X/Y Antibody (E-12) References:

1. Usp9y (ubiquitin-specific protease 9 gene on the Y) is associated with a functional promoter and encodes an intact open reading frame homologous to Usp9x that is under selective constraint.  |  Hall, NM., et al. 2003. Mamm Genome. 14: 437-47. PMID: 12925892
2. Doublecortin interacts with the ubiquitin protease DFFRX, which associates with microtubules in neuronal processes.  |  Friocourt, G., et al. 2005. Mol Cell Neurosci. 28: 153-64. PMID: 15607950
3. Spatially and temporally specific expression in mouse hippocampus of Usp9x, a ubiquitin-specific protease involved in synaptic development.  |  Xu, J., et al. 2005. J Neurosci Res. 80: 47-55. PMID: 15723417
4. Age-related changes in Usp9x protein expression and DNA methylation in mouse brain.  |  Xu, J. 2005. Brain Res Mol Brain Res. 140: 17-24. PMID: 16023255
5. 14-3-3 cooperates with LKB1 to regulate the activity and localization of QSK and SIK.  |  Al-Hakim, AK., et al. 2005. J Cell Sci. 118: 5661-73. PMID: 16306228
6. The ubiquitin ligase itch is auto-ubiquitylated in vivo and in vitro but is protected from degradation by interacting with the deubiquitylating enzyme FAM/USP9X.  |  Mouchantaf, R., et al. 2006. J Biol Chem. 281: 38738-47. PMID: 17038327
7. Control of AMPK-related kinases by USP9X and atypical Lys(29)/Lys(33)-linked polyubiquitin chains.  |  Al-Hakim, AK., et al. 2008. Biochem J. 411: 249-60. PMID: 18254724
8. USP9X deubiquitinates ALDH1A3 and maintains mesenchymal identity in glioblastoma stem cells.  |  Chen, Z., et al. 2019. J Clin Invest. 129: 2043-2055. PMID: 30958800
9. USP9X-mediated KDM4C deubiquitination promotes lung cancer radioresistance by epigenetically inducing TGF-β2 transcription.  |  Jie, X., et al. 2021. Cell Death Differ. 28: 2095-2111. PMID: 33558705
10. Disruption of USP9X in macrophages promotes foam cell formation and atherosclerosis.  |  Wang, B., et al. 2022. J Clin Invest. 132: PMID: 35389885
11. Cellular functions, molecular signalings and therapeutic applications: Translational potential of deubiquitylating enzyme USP9X as a drug target in cancer treatment.  |  Gao, H., et al. 2024. Biochim Biophys Acta Rev Cancer. 1879: 189099. PMID: 38582329
12. Characterisation of the coding sequence and fine mapping of the human DFFRY gene and comparative expression analysis and mapping to the Sxrb interval of the mouse Y chromosome of the Dffry gene.  |  Brown, GM., et al. 1998. Hum Mol Genet. 7: 97-107. PMID: 9384609

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-USP9X/Y Antibody (E-12) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-USP9X/Y Antibody (E-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

USP9X/Y Antibody (E-12) Specifications

Alternate Product Name: USP9X/Y

Product Clone Name: E-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 2248-2547

Epitope Target: USP9X/Y

Epitope Species: human

Product Reactivity/Detection: USP9X of mouse, rat and human orgin, and USP9Y of human origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for USP9X/Y Antibody (E-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin