USP9X CRISPR/Cas9 KO Plasmid (h): sc-402285
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- USP9X CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the USP9X genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USP9X CRISPR/Cas9 KO Plasmid (h) | sc-402285 | 20 µg | $397.00 |
Overview
USP9X encodes a ubiquitin-specific protease that regulates protein stability by removing ubiquitin chains from key substrates, shaping proteostasis and signaling amplitude. It has been linked to control of cell survival and stress responses through modulation of pathways such as NF-κB signaling, DNA damage response networks, and membrane trafficking-associated processes. By influencing turnover of regulatory proteins and components of the ubiquitin–proteasome system, USP9X impacts cell-cycle progression, apoptosis, and differentiation programs. Dysregulated USP9X activity or expression has been associated with altered signaling homeostasis in multiple disease-relevant contexts, including cancer biology and neurodevelopmental phenotypes, making it a useful target for mechanistic studies.
USP9X CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the USP9X gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the USP9X together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the USP9X open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish USP9X protein expression.
This CRISPR knockout system enables efficient generation of USP9X-deficient cell models for investigation of USP9X signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting USP9X exon(s) critical for USP9X function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple USP9X genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by USP9X CRISPR/Cas9 KO Plasmid (h) and USP9X CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the USP9X locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by USP9X HDR Plasmid (h) and USP9X HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by USP9X homology arms to support homology-directed repair at defined USP9X target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.