USP32 Double Nickase Plasmid (m): sc-433591-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- USP32 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- USP32 Double Nickase Plasmid (m) and USP32 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Usp32. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: USP32 Antibody (A-10): sc-374465
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USP32 Double Nickase Plasmid (m) | sc-433591-NIC | 20 µg | $410.00 |
Mouse USP32 (Usp32) encodes a ubiquitin-specific protease that regulates protein stability and signaling output by removing ubiquitin chains from selected substrates, thereby influencing proteostasis and the balance between recycling and degradation. As a deubiquitinating enzyme, USP32 is linked to ubiquitin-dependent control of receptor trafficking, endosomal dynamics, and downstream signaling pathways that coordinate cell growth and stress responses. Altered deubiquitination can perturb pathway timing and amplitude, contributing to dysregulated proliferation, aberrant survival signaling, and changes in cellular homeostasis. In biomedical research, Usp32 is therefore studied in the context of ubiquitin-system regulation, membrane-associated signaling networks, and mechanisms relevant to cancer biology and other disorders of proteostasis.
USP32 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Usp32 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Usp32. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Usp32 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Usp32-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.