USP28 CRISPR/Cas9 KO Plasmid (m): sc-433446
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- USP28 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the USP28 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USP28 CRISPR/Cas9 KO Plasmid (m) | sc-433446 | 20 µg | $397.00 |
Overview
Usp28 encodes ubiquitin-specific peptidase 28 (USP28), a deubiquitinating enzyme that edits ubiquitin chains to regulate protein stability and turnover. USP28 participates in ubiquitin-dependent quality control and signaling processes that influence DNA damage responses, cell-cycle progression, and transcriptional programs by controlling the abundance of key regulatory factors. Through these activities, USP28 is studied in pathways linked to genome stability, stress signaling, and proteostasis. Altered USP28 function has been associated with dysregulated proliferation and oncogenic signaling contexts, making it relevant for mechanistic studies in cancer biology and related cellular models.
USP28 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Usp28 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Usp28 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Usp28 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish USP28 protein expression.
This CRISPR knockout system enables efficient generation of Usp28-deficient cell models for investigation of USP28 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Usp28 exon(s) critical for USP28 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Usp28 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by USP28 CRISPR/Cas9 KO Plasmid (m) and USP28 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Usp28 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by USP28 HDR Plasmid (m) and USP28 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Usp28 homology arms to support homology-directed repair at defined Usp28 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.