UPIIIa CRISPR/Cas9 KO Plasmid (m): sc-423622
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UPIIIa CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UPIIIa genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UPIIIa CRISPR/Cas9 KO Plasmid (m) | sc-423622 | 20 µg | $397.00 |
Overview
Upk3a encodes uroplakin IIIa (UPIIIa), a tetraspanin-like component of the urothelial apical membrane that participates in assembling uroplakin plaques and maintaining the permeability barrier of the urinary tract. UPIIIa forms complexes with other uroplakins to support terminal differentiation of umbrella cells and to regulate membrane trafficking and mechanical stability during bladder filling and voiding. Disruption of uroplakin composition can compromise epithelial integrity, alter urothelial differentiation programs, and promote inflammatory responses linked to urinary tract dysfunction. In mouse models, Upk3a is used to interrogate pathways governing epithelial barrier formation, urothelial remodeling, and host–pathogen interactions at the bladder surface.
UPIIIa CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Upk3a gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Upk3a together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Upk3a open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UPIIIa protein expression.
This CRISPR knockout system enables efficient generation of Upk3a-deficient cell models for investigation of UPIIIa signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Upk3a exon(s) critical for UPIIIa function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Upk3a genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UPIIIa CRISPR/Cas9 KO Plasmid (m) and UPIIIa CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Upk3a locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UPIIIa HDR Plasmid (m) and UPIIIa HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Upk3a homology arms to support homology-directed repair at defined Upk3a target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.