UPII CRISPR/Cas9 KO Plasmid (m): sc-423621
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UPII CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UPII genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UPII CRISPR/Cas9 KO Plasmid (m) | sc-423621 | 20 µg | $397.00 |
Overview
Upk2 encodes uroplakin II (UPII), a tetraspanin-associated differentiation marker of urothelial umbrella cells that contributes to assembly of the apical urothelial plaque and maintenance of the urine–blood barrier. UPII participates in membrane trafficking and formation of uroplakin complexes that organize specialized lipid microdomains, supporting epithelial polarity and barrier function. Altered uroplakin composition or differentiation programs in the urothelium has been linked to disrupted barrier integrity, inflammation, and susceptibility to urinary tract injury. In mouse models, Upk2 expression is commonly used to study urothelial lineage identity and epithelial remodeling in bladder pathophysiology.
UPII CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Upk2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Upk2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Upk2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UPII protein expression.
This CRISPR knockout system enables efficient generation of Upk2-deficient cell models for investigation of UPII signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Upk2 exon(s) critical for UPII function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Upk2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UPII CRISPR/Cas9 KO Plasmid (m) and UPII CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Upk2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UPII HDR Plasmid (m) and UPII HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Upk2 homology arms to support homology-directed repair at defined Upk2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.