UPase CRISPR/Cas9 KO Plasmid (m): sc-423623
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UPase CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UPase genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UPase CRISPR/Cas9 KO Plasmid (m) | sc-423623 | 20 µg | $397.00 |
Overview
Mouse Upp1 encodes uridine phosphorylase (UPase), a key enzyme of the pyrimidine salvage pathway that catalyzes the reversible phosphorolysis of uridine to uracil and ribose-1-phosphate. By regulating cellular nucleoside pools, UPase influences RNA/DNA precursor availability and integrates with nucleotide metabolism programs that support proliferation and metabolic adaptation. Upp1 expression and UPase activity have been studied in contexts of tissue remodeling and stress responses where shifts in nucleoside turnover can alter cellular homeostasis. As a metabolic node, Upp1 is frequently used to interrogate links between pyrimidine metabolism, mitochondrial and redox balance, and pathway crosstalk affecting growth and survival phenotypes.
UPase CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Upp1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Upp1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Upp1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UPase protein expression.
This CRISPR knockout system enables efficient generation of Upp1-deficient cell models for investigation of UPase signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Upp1 exon(s) critical for UPase function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Upp1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UPase CRISPR/Cas9 KO Plasmid (m) and UPase CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Upp1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UPase HDR Plasmid (m) and UPase HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Upp1 homology arms to support homology-directed repair at defined Upp1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.