Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
uPAR Double Nickase Plasmid (h): sc-400666-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- uPAR Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- uPAR Double Nickase Plasmid (h) and uPAR Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PLAUR. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: uPAR Antibody (E-3): sc-376494
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
uPAR Double Nickase Plasmid (h) | sc-400666-NIC | 20 µg | $410.00 | |||
uPAR Double Nickase Plasmid (h2) | sc-400666-NIC-2 | 20 µg | $410.00 |
Human PLAUR encodes the urokinase plasminogen activator receptor (uPAR), a GPI-anchored cell-surface receptor that concentrates uPA activity to promote plasmin generation and pericellular proteolysis. Through interactions with vitronectin and co-receptors such as integrins and FPR family members, uPAR coordinates extracellular matrix remodeling, cell adhesion, migration, and invasion-related signaling, linking protease activity to cytoskeletal dynamics and MAPK/PI3K-associated pathways. Dysregulated PLAUR/uPAR expression has been associated with inflammatory remodeling and tumor microenvironment biology, where altered proteolysis and cell motility contribute to disease-associated phenotypes. As a nodal regulator at the cell surface, uPAR is frequently studied in mechanisms of metastatic dissemination, angiogenesis, and immune cell trafficking.
uPAR Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PLAUR locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PLAUR. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PLAUR function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PLAUR-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.