uPA/urokinase/PLAU Antibody (H77A10): sc-59727

uPA Antibody (H77A10) is a mouse monoclonal IgG1 kappa light chain antibody that specifically detects urokinase-type plasminogen activator (uPA), also known as PLAU or urokinase, in mouse, rat, and human samples by western blotting (WB), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). uPA monoclonal antibody (H77A10) was raised against a recombinant protein corresponding to the full-length, glycosylated uPA of mouse origin. uPA is a serine protease crucial for converting plasminogen to plasmin, playing a significant role in fibrinolysis and extracellular matrix degradation. uPA is primarily located on the cell surface, bound to its receptor uPAR, which localizes its proteolytic activity and facilitates processes like cell migration and tissue remodeling. The cell surface localization of uPA allows for targeted proteolysis, essential in wound healing, inflammation, and tumor metastasis. By interacting with uPAR, uPA influences cell signaling pathways that regulate cell adhesion, proliferation, and survival. The activity of uPA is tightly regulated by serine protease inhibitors PAI-1 and PAI-2, which inhibit uPA and control its role in physiological and pathological processes. Anti-uPA antibody (H77A10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

uPA/urokinase/PLAU Antibody (H77A10) References:

1. Binding of tissue plasminogen activator to human endothelial cells. Importance of the B-chain as a ligand.  |  Cheng, XF., et al. 1992. Biochem J. 287 (Pt 2): 407-13. PMID: 1332673
2. The human urokinase-plasminogen activator gene and its promoter.  |  Riccio, A., et al. 1985. Nucleic Acids Res. 13: 2759-71. PMID: 2987867
3. The human tissue plasminogen activator gene.  |  Degen, SJ., et al. 1986. J Biol Chem. 261: 6972-85. PMID: 3009482
4. Tissue-type plasminogen activator: a new fibrinolytic agent.  |  Milligan, KS. 1987. Heart Lung. 16: 69-74. PMID: 3098703
5. Tissue plasminogen activator.  |  Loscalzo, J. and Braunwald, E. 1988. N Engl J Med. 319: 925-31. PMID: 3138537
6. Differential expression of urokinase-type plasminogen activator (uPA), its receptor (uPA-R), and inhibitor type-2 (PAI-2) during differentiation of keratinocytes in an organotypic coculture system.  |  Schaefer, BM., et al. 1995. Exp Cell Res. 220: 415-23. PMID: 7556451
7. The fibrinolytic response to ancrod therapy: characterization of fibrinogen and fibrin degradation products.  |  Prentice, CR., et al. 1993. Br J Haematol. 83: 276-81. PMID: 8457476

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-uPA/urokinase/PLAU Antibody (H77A10) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-uPA/urokinase/PLAU Antibody (H77A10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

uPA/urokinase/PLAU Antibody (H77A10) Specifications

Alternate Product Name: uPA

Product Clone Name: H77A10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: uPA

Epitope Species: mouse

Product Reactivity/Detection: uPA

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for uPA/urokinase/PLAU Antibody (H77A10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin