UNKL Double Nickase Plasmid (h): sc-413020-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UNKL Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- UNKL Double Nickase Plasmid (h) and UNKL Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting UNKL. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UNKL Antibody (F-8): sc-398716
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UNKL Double Nickase Plasmid (h) | sc-413020-NIC | 20 µg | $410.00 | |||
UNKL Double Nickase Plasmid (h2) | sc-413020-NIC-2 | 20 µg | $410.00 |
UNKL encodes a poorly characterized human protein thought to participate in intracellular organization and membrane-associated processes, with emerging links to ubiquitin-dependent regulation of protein stability and trafficking. Based on predicted domains and limited functional reports, UNKL may influence proteostasis and signaling outputs by modulating the turnover or localization of specific client proteins. Altered regulation of these pathways can affect cell-state transitions, stress responses, and growth control, making UNKL a target of interest in systems-level studies of pathway wiring. Expression and genetic association datasets have suggested potential relevance to disease biology, including contexts where dysregulated protein homeostasis and signaling contribute to cellular dysfunction.
UNKL Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the UNKL locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within UNKL. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt UNKL function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of UNKL-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.