UNC5H3 Antibody (F-4): sc-515678

UNC5H3 Antibody (F-4) is a mouse monoclonal IgG2b antibody that detects UNC5H3 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). UNC5H3 (F-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UNC5H3, also known as UNC5C, is a member of the UNC5 family of proteins, which function as transmembrane receptors for netrin-1, playing a pivotal role in axon guidance and neural cell migration. UNC5H3′s structure contains a death domain crucial for pro-apoptotic function; when cleaved by caspases, this domain facilitates programmed cell death, a vital process in maintaining cellular homeostasis and preventing tumorigenesis. In the absence of netrin-1, UNC5H3 acts as a tumor suppressor by inhibiting anchorage-independent growth and invasion, highlighting UNC5H3′s significance in cancer biology. Expression of UNC5H3 is often down-regulated in various cancers, including colorectal, breast, ovarian, uterine, stomach, lung, and kidney cancers, indicating UNC5H3′s potential as a therapeutic target. UNC5H3 is essential for spinal accessory motor neuron development and mediates netrin-1′s repulsive action, serving as a critical stop signal for migratory cells during neural development.

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UNC5H3 Antibody (F-4) References:

1. Netrin-1 acts as a survival factor via its receptors UNC5H and DCC.  |  Llambi, F., et al. 2001. EMBO J. 20: 2715-22. PMID: 11387206
2. Modulation of G(ialpha(2)) signaling by the axonal guidance molecule UNC5H2.  |  Komatsuzaki, K., et al. 2002. Biochem Biophys Res Commun. 297: 898-905. PMID: 12359238
3. The netrin-1 receptors UNC5H are putative tumor suppressors controlling cell death commitment.  |  Thiebault, K., et al. 2003. Proc Natl Acad Sci U S A. 100: 4173-8. PMID: 12655055
4. Rat neurological mutations cerebellar vermis defect and hobble are caused by mutations in the netrin-1 receptor gene Unc5h3.  |  Kuramoto, T., et al. 2004. Brain Res Mol Brain Res. 122: 103-8. PMID: 15010202
5. Mapping netrin receptor binding reveals domains of Unc5 regulating its tyrosine phosphorylation.  |  Kruger, RP., et al. 2004. J Neurosci. 24: 10826-34. PMID: 15574733
6. RAR-related orphan receptor A isoform 1 (RORa1) is disrupted by a balanced translocation t(4;15)(q22.3;q21.3) associated with severe obesity.  |  Klar, J., et al. 2005. Eur J Hum Genet. 13: 928-34. PMID: 15886715
7. UNC5C is required for spinal accessory motor neuron development.  |  Dillon, AK., et al. 2007. Mol Cell Neurosci. 35: 482-9. PMID: 17543537
8. NSCL-1 and -2 control the formation of precerebellar nuclei by orchestrating the migration of neuronal precursor cells.  |  Schmid, T., et al. 2007. J Neurochem. 102: 2061-2072. PMID: 17573818
9. Analysis of Netrin 1 receptors during inner ear development.  |  Matilainen, T., et al. 2007. Int J Dev Biol. 51: 409-13. PMID: 17616930
10. FLRT2 and FLRT3 act as repulsive guidance cues for Unc5-positive neurons.  |  Yamagishi, S., et al. 2011. EMBO J. 30: 2920-33. PMID: 21673655
11. Structural Basis of Latrophilin-FLRT-UNC5 Interaction in Cell Adhesion.  |  Lu, YC., et al. 2015. Structure. 23: 1678-1691. PMID: 26235030
12. Structural Decoding of the Netrin-1/UNC5 Interaction and its Therapeutical Implications in Cancers.  |  Grandin, M., et al. 2016. Cancer Cell. 29: 173-85. PMID: 26859457

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-UNC5H3 Antibody (F-4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-UNC5H3 Antibody (F-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

UNC5H3 Antibody (F-4) Specifications

Alternate Product Name: UNC5H3

Product Clone Name: F-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids: 516-537

Epitope Target: UNC5H3

Epitope Species: human

Product Reactivity/Detection: UNC5H3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for UNC5H3 Antibody (F-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin