UHRF1 Double Nickase Plasmid (m): sc-421937-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UHRF1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- UHRF1 Double Nickase Plasmid (m) and UHRF1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Uhrf1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UHRF1 Antibody (H-8): sc-373750
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UHRF1 Double Nickase Plasmid (m) | sc-421937-NIC | 20 µg | $410.00 | |||
UHRF1 Double Nickase Plasmid (m2) | sc-421937-NIC-2 | 20 µg | $410.00 |
Uhrf1 encodes UHRF1, a multidomain epigenetic regulator that couples recognition of hemimethylated CpG DNA with recruitment of maintenance DNA methylation machinery during S phase. Through its SRA, PHD, and RING domains, UHRF1 coordinates DNA methylation inheritance, histone modification crosstalk, and chromatin remodeling to preserve genome stability and transcriptional programs. In mouse cells, UHRF1 activity is tightly linked to cell-cycle progression, replication-coupled chromatin assembly, and repression of transposable elements, with downstream effects on differentiation and lineage commitment. Dysregulated UHRF1-associated epigenetic maintenance is broadly relevant to oncogenic transformation, aberrant proliferation, and epigenome instability, making Uhrf1 a key node for mechanistic studies of methylation-driven gene regulation.
UHRF1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Uhrf1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Uhrf1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Uhrf1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Uhrf1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.