UHRF1 Antibody (H-8): sc-373750
- UHRF1 Antibody (H-8) is a mouse monoclonal IgG1 κ UHRF1 antibody, cited in 63 publications, provided at 200 µg/ml
- raised against amino acids 586-717 mapping near the C-terminus of UHRF1 of mouse origin
- UHRF1 Antibody (H-8) is recommended for detection of UHRF1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-UHRF1 Antibody (H-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for UHRF1 Antibody (H-8) for WB and IHC(P) applications. These reagents are now offered in bundles with UHRF1 Antibody (H-8) (see ordering information below).
QUICK LINKS
SEE ALSO...
UHRF1 Antibody (H-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects UHRF1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-UHRF1 antibody (H-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UHRF1, also known as ubiquitin-like, containing PHD and RING finger domains 1, Np95, ICBP90, or RNF106, is a crucial transcription and cell cycle regulator that belongs to the RING-finger type E3 ubiquitin ligase subfamily. UHRF1 is primarily localized in the nucleus, where UHRF1 plays a vital role in mediating the ubiquitination process, which is essential for protein degradation and cellular proliferation. UHRF1 contains a unique structure featuring one PHD-type zinc finger, a ubiquitin-like domain, two RING-type zinc fingers, and one YDG/SRA domain, which collectively facilitate its function as an E3 ubiquitin-protein ligase. UHRF1 directly interacts with Dnmt1, a maintenance DNA methyltransferase, ensuring stable association with chromatin, which is critical for the regulation of gene expression and epigenetic inheritance. Overexpression of UHRF1 in cancer cells highlights potential role in carcinogenesis, making UHRF1 a significant target for research in cancer biology and therapeutic interventions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
UHRF1 Antibody (H-8) References:
- ICBP90, a novel human CCAAT binding protein, involved in the regulation of topoisomerase IIalpha expression. | Hopfner, R., et al. 2000. Cancer Res. 60: 121-8. PMID: 10646863
- Np95 is regulated by E1A during mitotic reactivation of terminally differentiated cells and is essential for S phase entry. | Bonapace, IM., et al. 2002. J Cell Biol. 157: 909-14. PMID: 12058012
- Targeted disruption of Np95 gene renders murine embryonic stem cells hypersensitive to DNA damaging agents and DNA replication blocks. | Muto, M., et al. 2002. J Biol Chem. 277: 34549-55. PMID: 12084726
- The retinoblastoma gene and its product are targeted by ICBP90: a key mechanism in the G1/S transition during the cell cycle. | Jeanblanc, M., et al. 2005. Oncogene. 24: 7337-45. PMID: 16007129
- Critical role of the ubiquitin ligase activity of UHRF1, a nuclear RING finger protein, in tumor cell growth. | Jenkins, Y., et al. 2005. Mol Biol Cell. 16: 5621-9. PMID: 16195352
- UHRF1 plays a role in maintaining DNA methylation in mammalian cells. | Bostick, M., et al. 2007. Science. 317: 1760-4. PMID: 17673620
- ICBP90, a novel methyl K9 H3 binding protein linking protein ubiquitination with heterochromatin formation. | Karagianni, P., et al. 2008. Mol Cell Biol. 28: 705-17. PMID: 17967883
- A concern regarding the current confusion with the human homolog of mouse Np95, ICBP90/UHRF1. | Unoki, M., et al. 2008. Radiat Res. 169: 240-4. PMID: 18220474
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UHRF1 Antibody (H-8) | sc-373750 | 200 µg/ml | $322.00 | |||
UHRF1 Antibody (H-8): m-IgG Fc BP-HRP Bundle | sc-527410 | 200 µg Ab; 10 µg BP | $361.00 | |||
UHRF1 Antibody (H-8): m-IgG1 BP-HRP Bundle | sc-532783 | 200 µg Ab; 20 µg BP | $361.00 | |||
UHRF1 Antibody (H-8) AC | sc-373750 AC | 500 µg/ml, 25% agarose | $424.00 | |||
UHRF1 Antibody (H-8) HRP | sc-373750 HRP | 200 µg/ml | $322.00 | |||
UHRF1 Antibody (H-8) FITC | sc-373750 FITC | 200 µg/ml | $336.00 | |||
UHRF1 Antibody (H-8) PE | sc-373750 PE | 200 µg/ml | $349.00 | |||
UHRF1 Antibody (H-8) Alexa Fluor® 488 | sc-373750 AF488 | 200 µg/ml | $364.00 | |||
UHRF1 Antibody (H-8) Alexa Fluor® 546 | sc-373750 AF546 | 200 µg/ml | $364.00 | |||
UHRF1 Antibody (H-8) Alexa Fluor® 594 | sc-373750 AF594 | 200 µg/ml | $364.00 | |||
UHRF1 Antibody (H-8) Alexa Fluor® 647 | sc-373750 AF647 | 200 µg/ml | $364.00 | |||
UHRF1 Antibody (H-8) Alexa Fluor® 680 | sc-373750 AF680 | 200 µg/ml | $364.00 | |||
UHRF1 Antibody (H-8) Alexa Fluor® 790 | sc-373750 AF790 | 200 µg/ml | $364.00 |