UGT2B5 CRISPR/Cas9 KO Plasmid (m): sc-423603
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UGT2B5 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UGT2B5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UGT2B5 CRISPR/Cas9 KO Plasmid (m) | sc-423603 | 20 µg | $397.00 |
Overview
Mouse UGT2B5 is a microsomal UDP-glucuronosyltransferase that catalyzes glucuronic acid conjugation of lipophilic substrates, increasing their aqueous solubility for elimination. This phase II metabolism activity supports xenobiotic clearance and contributes to homeostatic control of endogenous small molecules through glucuronidation. UGT2B family enzymes interface with hepatic and extrahepatic detoxification programs and can shape cellular responses to chemical exposure by modulating substrate availability and metabolite profiles. Altered glucuronidation capacity is frequently investigated in the context of toxicology, inflammation-linked metabolic rewiring, and genotype-dependent variability in drug handling and chemical sensitivity.
UGT2B5 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ugt2b5 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ugt2b5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ugt2b5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UGT2B5 protein expression.
This CRISPR knockout system enables efficient generation of Ugt2b5-deficient cell models for investigation of UGT2B5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ugt2b5 exon(s) critical for UGT2B5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ugt2b5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UGT2B5 CRISPR/Cas9 KO Plasmid (m) and UGT2B5 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ugt2b5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UGT2B5 HDR Plasmid (m) and UGT2B5 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ugt2b5 homology arms to support homology-directed repair at defined Ugt2b5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.