UGT1A2 CRISPR/Cas9 KO Plasmid (m): sc-423602
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UGT1A2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UGT1A2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UGT1A2 CRISPR/Cas9 KO Plasmid (m) | sc-423602 | 20 µg | $397.00 |
Overview
Mouse Ugt1a2 encodes UDP-glucuronosyltransferase 1A2 (UGT1A2), a microsomal phase II conjugating enzyme that catalyzes glucuronidation of diverse endogenous metabolites and xenobiotics. By transferring glucuronic acid to lipophilic substrates, UGT1A2 increases solubility and promotes clearance through hepatic and extrahepatic detoxification pathways. Ugt1a2 activity contributes to the broader UGT1A-dependent network that modulates chemical disposition, oxidative stress responses, and interactions with cytochrome P450-driven metabolism. Genetic or experimental perturbation of UGT-mediated conjugation is relevant to studies of altered pharmacokinetics, susceptibility to chemical injury, and metabolism-linked phenotypes in mouse disease models.
UGT1A2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ugt1a2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ugt1a2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ugt1a2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UGT1A2 protein expression.
This CRISPR knockout system enables efficient generation of Ugt1a2-deficient cell models for investigation of UGT1A2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ugt1a2 exon(s) critical for UGT1A2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ugt1a2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UGT1A2 CRISPR/Cas9 KO Plasmid (m) and UGT1A2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ugt1a2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UGT1A2 HDR Plasmid (m) and UGT1A2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ugt1a2 homology arms to support homology-directed repair at defined Ugt1a2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.