UGCG Double Nickase Plasmid (m): sc-423600-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UGCG Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- UGCG Double Nickase Plasmid (m) and UGCG Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ugcg. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UGCG Antibody (1E5): sc-293235
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UGCG Double Nickase Plasmid (m) | sc-423600-NIC | 20 µg | $410.00 |
Mouse Ugcg encodes UDP-glucose ceramide glucosyltransferase (UGCG), a Golgi-resident enzyme that catalyzes the first glycosylation step converting ceramide to glucosylceramide, thereby initiating glycosphingolipid biosynthesis. Through control of cellular ceramide pools and downstream ganglioside and globoside production, UGCG influences membrane microdomain composition, vesicular trafficking, and receptor-mediated signaling. Altered UGCG activity perturbs sphingolipid homeostasis and has been linked to defects in lipid metabolism, immune and inflammatory signaling, and changes in cell stress responses relevant to metabolic and neurobiological phenotypes. As a pathway gatekeeper, UGCG is widely studied for its role in modulating apoptosis sensitivity, differentiation programs, and membrane-dependent signaling networks.
UGCG Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ugcg locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ugcg. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ugcg function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ugcg-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.