UFD1 Double Nickase Plasmid (h): sc-403304-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UFD1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- UFD1 Double Nickase Plasmid (h) and UFD1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting UFD1L. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UFD1 Antibody (B-7): sc-377265
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UFD1 Double Nickase Plasmid (h) | sc-403304-NIC | 20 µg | $410.00 | |||
UFD1 Double Nickase Plasmid (h2) | sc-403304-NIC-2 | 20 µg | $410.00 |
UFD1L encodes UFD1, a core component of the UFD1–NPL4 complex that cooperates with the p97/VCP AAA+ ATPase to extract polyubiquitinated substrates from membranes and macromolecular assemblies for proteasomal degradation. This axis is central to endoplasmic reticulum–associated degradation (ERAD), protein quality control, and ubiquitin-dependent turnover during cell-cycle progression and DNA damage responses. By regulating clearance of misfolded or stalled protein complexes, UFD1 supports proteostasis and stress adaptation pathways that influence viability in rapidly proliferating cells. Altered regulation of the p97/VCP–UFD1/NPL4 pathway has been linked to proteostasis imbalance and genome maintenance defects relevant to studies of neurodegeneration and cancer-associated cellular phenotypes.
UFD1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the UFD1L locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within UFD1L. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt UFD1L function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of UFD1L-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.