UDG Antibody (k1C12): sc-73639
- UDG Antibody (k1C12) is a mouse monoclonal IgG2b, cited in 1 publications, provided at 50 µg/0.5 ml
- raised against amino acids 1-313 of UDG of human origin
- recommended for detection of UDG of human origin by WB and IP
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for UDG Antibody (k1C12). This work is in progress.
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SEE ALSO...
UDG Antibody (k1C12) is a mouse monoclonal IgG2b kappa light chain antibody that detects uracil-DNA glycosylase (UDG) in human samples through western blotting (WB) and immunoprecipitation (IP) applications. UDG plays a crucial role in the DNA repair process by excising uracil from DNA, which can arise from misincorporation or cytosine deamination. This function is vital for maintaining genomic integrity, as the presence of uracil in DNA can lead to mutations and genomic instability if not repaired. UDG operates by cleaving the N-C1′ glycosidic bond between the uracil base and deoxyribose, transitioning from an "open" unbound state to a "closed" DNA-bound state, which creates a catalytic center essential for enzymatic activity. The conformational change is facilitated by a B zipper motif within UDG, allowing effective flipping of the uridine nucleotide into the catalytic center for excision. Notably, UDG exists in two isoforms, UDG1 and UDG1A, with UDG1 localized to the mitochondria and UDG1A, a processed isoform, localized to the nucleus due to a unique 44-residue amino-terminus. Anti-UDG antibody (k1C12) serves as an invaluable tool for researchers studying DNA repair mechanisms and the implications of uracil misincorporation in human health.
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LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
UDG Antibody (k1C12) References:
- Monitoring eukaryotic and bacterial UDG repair activity with DNA-multifluorophore sensors. | Ono, T., et al. 2013. Nucleic Acids Res. 41: e127. PMID: 23644286
- A novel combined bisulfite UDG assay for selective 5-methylcytosine detection. | Huang, R., et al. 2013. Talanta. 117: 445-8. PMID: 24209366
- Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP). | Hsieh, K., et al. 2014. Chem Commun (Camb). 50: 3747-9. PMID: 24577617
- High UDG and BRCA1 expression is associated with adverse outcome in patients with pemetrexed treated non-small cell lung Cancer. | Hashemi Sadraei, N., et al. 2018. Lung Cancer. 126: 48-54. PMID: 30527192
- A tri-functional probe mediated exponential amplification strategy for highly sensitive detection of Dnmt1 and UDG activities at single-cell level. | Fan, L., et al. 2020. Anal Chim Acta. 1103: 164-173. PMID: 32081181
- Smart Catalyzed Hairpin Assembly-Induced DNAzyme Nanosystem for Intracellular UDG Imaging. | Song, X., et al. 2021. Anal Chem. 93: 13687-13693. PMID: 34583508
- Colorimetric detection of SARS-CoV-2 by uracil-DNA glycosylase (UDG) reverse transcription loop-mediated isothermal amplification (RT-LAMP). | Lai, MY., et al. 2022. Int J Infect Dis. 120: 132-134. PMID: 35472524
- Target-manipulated drawstring DNAzyme for ultrasensitive detection of UDG using Au@Ag NRs indicator. | Li, J., et al. 2022. Anal Chim Acta. 1218: 340010. PMID: 35701040
- Circ-Udg Derived from Cyprinid Herpesvirus 2 Promotes Viral Replication. | Zhu, M., et al. 2022. Microbiol Spectr. 10: e0094322. PMID: 35770986
- One-step sensitive detection of UDG activity based on nicking enzyme-assisted signal amplification coupled with APE1 and triggered reporter. | Lai, S., et al. 2025. Anal Chim Acta. 1345: 343686. PMID: 40015794
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UDG Antibody (k1C12) | sc-73639 | 50 µg/0.5 ml | $322.00 |