UCP3 CRISPR/Cas9 KO Plasmid (m): sc-423597
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UCP3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UCP3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UCP3 CRISPR/Cas9 KO Plasmid (m) | sc-423597 | 20 µg | $397.00 |
Overview
Ucp3 encodes mitochondrial uncoupling protein 3 (UCP3), an inner membrane carrier that modulates proton leak and coupling efficiency during oxidative phosphorylation. In mouse skeletal muscle and brown adipose tissue, UCP3 is linked to fatty acid utilization, regulation of mitochondrial reactive oxygen species, and adaptive thermogenic responses to changes in nutrient availability. By influencing mitochondrial bioenergetics and lipid handling, UCP3 participates in pathways relevant to energy homeostasis, insulin sensitivity, and oxidative stress. Altered Ucp3 expression or activity is frequently examined in models of obesity, metabolic syndrome, and muscle metabolic adaptation.
UCP3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ucp3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ucp3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ucp3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UCP3 protein expression.
This CRISPR knockout system enables efficient generation of Ucp3-deficient cell models for investigation of UCP3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ucp3 exon(s) critical for UCP3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ucp3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UCP3 CRISPR/Cas9 KO Plasmid (m) and UCP3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ucp3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UCP3 HDR Plasmid (m) and UCP3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ucp3 homology arms to support homology-directed repair at defined Ucp3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.