UCP2 Double Nickase Plasmid (h): sc-400319-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UCP2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- UCP2 Double Nickase Plasmid (h) and UCP2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting UCP2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UCP2 Antibody (G-6): sc-390189
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UCP2 Double Nickase Plasmid (h) | sc-400319-NIC | 20 µg | $410.00 | |||
UCP2 Double Nickase Plasmid (h2) | sc-400319-NIC-2 | 20 µg | $410.00 |
UCP2 encodes mitochondrial uncoupling protein 2, an inner membrane carrier that modulates proton leak to tune oxidative phosphorylation efficiency and mitochondrial membrane potential. By shaping ROS production, NADH/NAD⁺ balance, and substrate utilization, UCP2 influences bioenergetic and redox-linked pathways including mitochondrial stress responses, inflammasome signaling, and insulin secretion dynamics. Altered UCP2 activity has been associated with metabolic dysregulation, immune-cell activation states, and cancer cell survival phenotypes where mitochondrial coupling and oxidative stress tolerance are selective pressures. As a result, UCP2 is frequently studied in the context of mitochondrial function, oxidative stress biology, and metabolic reprogramming.
UCP2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the UCP2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within UCP2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt UCP2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of UCP2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.