Ubr2 Antibody (8H10): sc-135594

Ubr2 Antibody (8H10) is a mouse monoclonal IgG2a kappa light chain antibody that detects Ubr2 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Ubr2 antibody (8H10) is available as a non-conjugated format. Ubr2, also known as Ubiquitin-protein ligase E3-alpha-2 or N-recognin-2, is a crucial component of the ubiquitin-proteasome system, which is essential for maintaining cellular homeostasis by regulating protein degradation. This 1755 amino acid protein features one UBR-type zinc finger and one RING-type zinc finger, which are vital for its function as an E3 ubiquitin-protein ligase. Ubr2 specifically recognizes proteins with destabilizing N-terminal residues, facilitating their ubiquitination and subsequent degradation, a process that is particularly important for controlling the levels of short-lived or abnormal proteins within the cell. The proper functioning of Ubr2 is critical, as evidenced by studies showing that mice lacking Ubr2 are infertile due to defects in male meiosis, highlighting Ubr2′s role in reproductive biology.

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Ubr2 Antibody (8H10) References:

1. A family of mammalian E3 ubiquitin ligases that contain the UBR box motif and recognize N-degrons.  |  Tasaki, T., et al. 2005. Mol Cell Biol. 25: 7120-36. PMID: 16055722
2. Loss of Ubr2, an E3 ubiquitin ligase, leads to chromosome fragility and impaired homologous recombinational repair.  |  Ouyang, Y., et al. 2006. Mutat Res. 596: 64-75. PMID: 16488448
3. Impaired neurogenesis and cardiovascular development in mice lacking the E3 ubiquitin ligases UBR1 and UBR2 of the N-end rule pathway.  |  An, JY., et al. 2006. Proc Natl Acad Sci U S A. 103: 6212-7. PMID: 16606826
4. Synthetic heterovalent inhibitors targeting recognition E3 components of the N-end rule pathway.  |  Lee, MJ., et al. 2008. Proc Natl Acad Sci U S A. 105: 100-5. PMID: 18162545
5. The substrate recognition domains of the N-end rule pathway.  |  Tasaki, T., et al. 2009. J Biol Chem. 284: 1884-95. PMID: 19008229
6. UBR2 mediates transcriptional silencing during spermatogenesis via histone ubiquitination.  |  An, JY., et al. 2010. Proc Natl Acad Sci U S A. 107: 1912-7. PMID: 20080676
7. Role of N-end rule ubiquitin ligases UBR1 and UBR2 in regulating the leucine-mTOR signaling pathway.  |  Kume, K., et al. 2010. Genes Cells. 15: 339-49. PMID: 20298436
8. UBR2 of the N-end rule pathway is required for chromosome stability via histone ubiquitylation in spermatocytes and somatic cells.  |  An, JY., et al. 2012. PLoS One. 7: e37414. PMID: 22616001
9. The E3 ligase UBR2 regulates cell death under caspase deficiency via Erk/MAPK pathway.  |  Villa, E., et al. 2020. Cell Death Dis. 11: 1041. PMID: 33288741
10. UBR2 targets myosin heavy chain IIb and IIx for degradation: Molecular mechanism essential for cancer-induced muscle wasting.  |  Gao, S., et al. 2022. Proc Natl Acad Sci U S A. 119: e2200215119. PMID: 36252004
11. The phosphatase DUSP22 inhibits UBR2-mediated K63-ubiquitination and activation of Lck downstream of TCR signalling.  |  Shih, YC., et al. 2024. Nat Commun. 15: 532. PMID: 38225265
12. N-recognins UBR1 and UBR2 as central ER stress sensors in mammals.  |  Le, LTHL., et al. 2024. Mol Cells. 47: 100001. PMID: 38376480