UBE2G2 Double Nickase Plasmid (h): sc-404851-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UBE2G2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- UBE2G2 Double Nickase Plasmid (h) and UBE2G2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting UBE2G2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UBE2G2 Antibody (D-4): sc-393780
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBE2G2 Double Nickase Plasmid (h) | sc-404851-NIC | 20 µg | $410.00 | |||
UBE2G2 Double Nickase Plasmid (h2) | sc-404851-NIC-2 | 20 µg | $410.00 |
UBE2G2 encodes an E2 ubiquitin-conjugating enzyme that collaborates with E3 ligases to assemble ubiquitin chains and regulate proteasome-dependent protein turnover. It is a key component of endoplasmic reticulum–associated degradation (ERAD), supporting the clearance of misfolded or surplus proteins and maintaining proteostasis under cellular stress. Through ubiquitination-dependent control of substrate stability, UBE2G2 influences pathways tied to the unfolded protein response, cell cycle regulation, and signaling amplitude. Dysregulated ubiquitin–proteasome and ERAD activity has been linked to cancer biology, neurodegeneration, and immune signaling perturbations, making UBE2G2 a useful node for mechanistic studies of protein quality control.
UBE2G2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the UBE2G2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within UBE2G2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt UBE2G2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of UBE2G2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.