UBE2E2 CRISPR/Cas9 KO Plasmid (h): sc-406437
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UBE2E2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UBE2E2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBE2E2 CRISPR/Cas9 KO Plasmid (h) | sc-406437 | 20 µg | $397.00 |
Overview
UBE2E2 encodes an E2 ubiquitin-conjugating enzyme that transfers activated ubiquitin from E1 enzymes to E3 ligases, supporting substrate ubiquitination and proteostasis. Through its role in the ubiquitin–proteasome system, UBE2E2 influences protein turnover, stress responses, and the regulation of signaling nodes that depend on controlled ubiquitination. Altered ubiquitination capacity can affect pathways governing cell-cycle progression and DNA damage responses, linking UBE2E2 dysfunction to phenotypes relevant to oncogenic signaling and cellular homeostasis. UBE2E2 is therefore frequently studied as a modulator of ubiquitin-dependent regulation and as a component of broader protein quality-control networks.
UBE2E2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the UBE2E2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the UBE2E2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the UBE2E2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UBE2E2 protein expression.
This CRISPR knockout system enables efficient generation of UBE2E2-deficient cell models for investigation of UBE2E2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting UBE2E2 exon(s) critical for UBE2E2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple UBE2E2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UBE2E2 CRISPR/Cas9 KO Plasmid (h) and UBE2E2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the UBE2E2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UBE2E2 HDR Plasmid (h) and UBE2E2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by UBE2E2 homology arms to support homology-directed repair at defined UBE2E2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.