UBE2E1 CRISPR/Cas9 KO Plasmid (m): sc-423577
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UBE2E1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UBE2E1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBE2E1 CRISPR/Cas9 KO Plasmid (m) | sc-423577 | 20 µg | $397.00 |
Overview
Ube2e1 encodes UBE2E1, a ubiquitin-conjugating E2 enzyme that cooperates with E3 ligases to transfer ubiquitin onto substrate proteins, shaping proteostasis and protein turnover. Through its role in ubiquitination, UBE2E1 contributes to regulation of cell-cycle progression, DNA damage responses, and stress-adaptive signaling by modulating the stability and activity of key regulatory proteins. Perturbation of E2 enzyme activity can disrupt ubiquitin-dependent quality control and signaling networks, processes frequently implicated in neurodegeneration, inflammation, and oncogenic transformation. In mouse models, Ube2e1 provides a tractable node for dissecting ubiquitin pathway specificity and redundancy among E2 family members in tissue and disease-relevant contexts.
UBE2E1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ube2e1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ube2e1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ube2e1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UBE2E1 protein expression.
This CRISPR knockout system enables efficient generation of Ube2e1-deficient cell models for investigation of UBE2E1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ube2e1 exon(s) critical for UBE2E1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ube2e1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UBE2E1 CRISPR/Cas9 KO Plasmid (m) and UBE2E1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ube2e1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UBE2E1 HDR Plasmid (m) and UBE2E1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ube2e1 homology arms to support homology-directed repair at defined Ube2e1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.