UBE2A/B Antibody (G-9): sc-365507
- UBE2A/B Antibody (G-9) is a mouse monoclonal IgG1 κ UBE2A/B antibody provided at 200 µg/ml
- raised against amino acids 1-75 mapping at the N-terminus of UBE2A of human origin
- UBE2A/B Antibody (G-9) is recommended for detection of UBE2A and UBE2B of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-UBE2A/B Antibody (G-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for UBE2A/B Antibody (G-9) for WB and IHC(P) applications. These reagents are now offered in bundles with UBE2A/B Antibody (G-9) (see ordering information below).
UBE2A/B Antibody (G-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects UBE2A/B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-UBE2A/B antibody (G-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UBE2A and UBE2B are crucial components of the ubiquitin-proteasome system, which plays a vital role in regulating protein degradation and cellular homeostasis. These ubiquitin-conjugating enzymes are primarily located in the nucleus and at the cell membrane, where UBE2A/B facilitate the postreplication repair of UV-damaged DNA. The localization of UBE2A and UBE2B is significant because these proteins interact with key proteins involved in DNA repair processes, such as Rad18, thereby ensuring genome integrity and preventing mutations that could lead to cancer. Using UBE2A/B monoclonal antibody (G-9) in research can provide insights into DNA repair mechanisms and ubiquitin signaling in cellular stress responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
UBE2A/B Antibody (G-9) References:
- Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway. | Gong, L. and Yeh, ET. 1999. J Biol Chem. 274: 12036-42. PMID: 10207026
- Heat-induced cell cycle arrest of Saccharomyces cerevisiae: involvement of the RAD6/UBC2 and WSC2 genes in its reversal. | Raboy, B., et al. 1999. Mol Microbiol. 32: 729-39. PMID: 10361277
- UBC13, a DNA-damage-inducible gene, is a member of the error-free postreplication repair pathway in Saccharomyces cerevisiae. | Brusky, J., et al. 2000. Curr Genet. 37: 168-74. PMID: 10794173
- Isolation and characterization of HRT1 using a genetic screen for mutants unable to degrade Gic2p in saccharomyces cerevisiae. | Blondel, M., et al. 2000. Genetics. 155: 1033-44. PMID: 10880467
- Ubiquitin, proteasomes, and the regulation of intracellular protein degradation. | Hochstrasser, M. 1995. Curr Opin Cell Biol. 7: 215-23. PMID: 7612274
- The ubiquitin-proteasome proteolytic pathway. | Ciechanover, A. 1994. Cell. 79: 13-21. PMID: 7923371
- The ubiquitin-mediated proteolytic pathway: mechanisms of recognition of the proteolytic substrate and involvement in the degradation of native cellular proteins. | Ciechanover, A. and Schwartz, AL. 1994. FASEB J. 8: 182-91. PMID: 8119489
- SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. | Bai, C., et al. 1996. Cell. 86: 263-74. PMID: 8706131
- The ubiquitin-like proteins SMT3 and SUMO-1 are conjugated by the UBC9 E2 enzyme. | Schwarz, SE., et al. 1998. Proc Natl Acad Sci U S A. 95: 560-4. PMID: 9435231
- A novel protein modification pathway related to the ubiquitin system. | Liakopoulos, D., et al. 1998. EMBO J. 17: 2208-14. PMID: 9545234
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBE2A/B Antibody (G-9) | sc-365507 | 200 µg/ml | $322.00 | |||
UBE2A/B Antibody (G-9): m-IgG Fc BP-HRP Bundle | sc-527374 | 200 µg Ab; 10 µg BP | $361.00 | |||
UBE2A/B Antibody (G-9): m-IgG1 BP-HRP Bundle | sc-532747 | 200 µg Ab; 20 µg BP | $361.00 | |||
UBE2A/B Antibody (G-9) AC | sc-365507 AC | 500 µg/ml, 25% agarose | $424.00 | |||
UBE2A/B Antibody (G-9) HRP | sc-365507 HRP | 200 µg/ml | $322.00 | |||
UBE2A/B Antibody (G-9) FITC | sc-365507 FITC | 200 µg/ml | $336.00 | |||
UBE2A/B Antibody (G-9) PE | sc-365507 PE | 200 µg/ml | $349.00 | |||
UBE2A/B Antibody (G-9) Alexa Fluor® 488 | sc-365507 AF488 | 200 µg/ml | $364.00 | |||
UBE2A/B Antibody (G-9) Alexa Fluor® 546 | sc-365507 AF546 | 200 µg/ml | $364.00 | |||
UBE2A/B Antibody (G-9) Alexa Fluor® 594 | sc-365507 AF594 | 200 µg/ml | $364.00 | |||
UBE2A/B Antibody (G-9) Alexa Fluor® 647 | sc-365507 AF647 | 200 µg/ml | $364.00 | |||
UBE2A/B Antibody (G-9) Alexa Fluor® 680 | sc-365507 AF680 | 200 µg/ml | $364.00 | |||
UBE2A/B Antibody (G-9) Alexa Fluor® 790 | sc-365507 AF790 | 200 µg/ml | $364.00 |