UBE1 Antibody (B-5): sc-271821
- UBE1 Antibody (B-5) is a mouse monoclonal IgM κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 99-131 near the N-terminus of UBE1 of human origin
- recommended for detection of UBE1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- See UBE1 (2G2): sc-53555 for UBE1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for UBE1 Antibody (B-5). This work is in progress.
UBE1 Antibody (B-5) is a mouse monoclonal IgM antibody that detects ubiquitin activating enzyme E1 (UBE1) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). UBE1 plays a crucial role in the ubiquitin-proteasome pathway, which is essential for regulating protein degradation and maintaining cellular homeostasis. By catalyzing the first step in ubiquitin conjugation, UBE1 activates ubiquitin through adenylation of its C-terminal glycine residue, forming a thiolester bond with a cysteine residue in UBE1 itself. This activation is ATP-dependent and is vital for the subsequent transfer of ubiquitin to ubiquitin-conjugating enzymes, which then attach ubiquitin to target substrates for degradation. The importance of UBE1 extends beyond its enzymatic function; UBE1 is also an X-linked gene that exhibits unique characteristics in humans, such as escaping X inactivation, which may have implications for sex-linked genetic disorders. Anti-UBE1 antibody (B-5) is an invaluable tool for researchers studying the dynamics of protein degradation and the intricate mechanisms of cellular regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
UBE1 Antibody (B-5) References:
- Expression, purification and characterization of human ubiquitin-activating enzyme, UBE1. | Zheng, M., et al. 2010. Mol Biol Rep. 37: 1413-9. PMID: 19343538
- The ubiquitin-activating enzyme E1 as a therapeutic target for the treatment of leukemia and multiple myeloma. | Xu, GW., et al. 2010. Blood. 115: 2251-9. PMID: 20075161
- Largazole and its derivatives selectively inhibit ubiquitin activating enzyme (e1). | Ungermannova, D., et al. 2012. PLoS One. 7: e29208. PMID: 22279528
- Ubiquitin-activating enzyme E1 inhibitor PYR41 attenuates angiotensin II-induced activation of dendritic cells via the IκBa/NF-κB and MKP1/ERK/STAT1 pathways. | Chen, C., et al. 2014. Immunology. 142: 307-19. PMID: 24456201
- Expression, purification, and crystal structure of N-terminal domains of human ubiquitin-activating enzyme (E1). | Xie, ST. 2014. Biosci Biotechnol Biochem. 78: 1542-9. PMID: 25209502
- Hydrophobic Patch of Ubiquitin is Important for its Optimal Activation by Ubiquitin Activating Enzyme E1. | Singh, RK., et al. 2017. Anal Chem. 89: 7852-7860. PMID: 28686836
- Human ubiquitin-activating enzyme, E1. Indication of potential nuclear and cytoplasmic subpopulations using epitope-tagged cDNA constructs. | Handley-Gearhart, PM., et al. 1994. J Biol Chem. 269: 33171-8. PMID: 7528747
- Nuclear localization of the ubiquitin-activating enzyme, E1, is cell-cycle-dependent. | Grenfell, SJ., et al. 1994. Biochem J. 300 (Pt 3): 701-8. PMID: 8010951
- Immunofluorescent localization of the ubiquitin-activating enzyme, E1, to the nucleus and cytoskeleton. | Trausch, JS., et al. 1993. Am J Physiol. 264: C93-102. PMID: 8430776
- The ubiquitin-activating enzyme E1 is phosphorylated and localized to the nucleus in a cell cycle-dependent manner. | Stephen, AG., et al. 1996. J Biol Chem. 271: 15608-14. PMID: 8663123
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBE1 Antibody (B-5) | sc-271821 | 200 µg/ml | $322.00 | |||
UBE1 (B-5) Neutralizing Peptide | sc-271821 P | 100 µg/0.5 ml | $69.00 |