Date published: 2025-12-18

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UBC12 Antibody (L-34): sc-100608

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Datasheets
  • UBC12 Antibody (L-34) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 100 µg/ml
  • raised against recombinant UBC12 of human origin
  • recommended for detection of UBC12 of human origin by WB, IP, IF and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for UBC12 Antibody (L-34). This work is in progress.

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UBC12 Antibody (L-34) is a mouse monoclonal IgG1 antibody that detects UBC12 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). UBC12, also known as UBE2M, is a 183 amino acid member of the E2 ubiquitin-conjugating enzyme family, playing a crucial role in the ubiquitination process, which is essential for regulating protein degradation and maintaining cellular homeostasis. UBC12 (L-34) monoclonal antibody interacts with NEDD8, a ubiquitin-like protein, to facilitate the attachment of NEDD8 to target proteins, thereby influencing various cellular processes, including cell cycle progression and signal transduction. The ability of UBC12 to modulate the conjugation of NEDD8 is vital for controlling the degradation of proteins that are critical for cell proliferation and differentiation. Anti-UBC12 antibody (L-34) enables researchers to gain insights into the mechanisms of protein regulation and the implications of NEDD8 modification in various biological contexts.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

UBC12 Antibody (L-34)

sc-100608
100 µg/ml
$333.00

I am using UBC12 (L-34): sc-100608 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jerojero
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Great monoclonal antibodyStrong band observed with little to no background with UBC12 Antibody (L-34)
Date published: 2017-02-08
Rated 5 out of 5 by from WB publication with human THPWB publication with human THP-1 cells.
Date published: 2015-06-11
Rated 5 out of 5 by from Worked well for Western blot with human transfectedWorked well for Western blot with human transfected lysates. -SCBT QC
Date published: 2015-05-09
Rated 5 out of 5 by from Beautiful immunofluorescence nuclear stainingBeautiful immunofluorescence nuclear staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-05-03
Rated 5 out of 5 by from Positive Western blot data obtained usingPositive Western blot data obtained using Ramos whole cell lysates. -SCBT QC
Date published: 2015-04-12
Rated 5 out of 5 by from Worked great for WB with Jurkat whole cell lysatesWorked great for WB with Jurkat whole cell lysates. -SCBT QC
Date published: 2015-03-04
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UBC12 Antibody (L-34) is rated 5.0 out of 5 by 6.
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