UBAC2 CRISPR/Cas9 KO Plasmid (h): sc-407388
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UBAC2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UBAC2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBAC2 CRISPR/Cas9 KO Plasmid (h) | sc-407388 | 20 µg | $397.00 |
Overview
UBAC2 (ubiquitin-associated domain containing 2) encodes an ER-associated protein implicated in ubiquitin-dependent protein quality control and proteostasis. Through its ubiquitin-binding features and subcellular localization, UBAC2 has been linked to regulation of ER stress responses and turnover of misfolded proteins, intersecting with pathways such as ER-associated degradation and broader ubiquitin–proteasome signaling. Genetic studies have associated UBAC2 loci with immune-related phenotypes, including susceptibility signals reported in inflammatory and autoimmune conditions, supporting its relevance to immunobiology. These connections make UBAC2 a useful target for dissecting how ubiquitin signaling modulates stress adaptation and immune-associated cellular states.
UBAC2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the UBAC2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the UBAC2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the UBAC2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UBAC2 protein expression.
This CRISPR knockout system enables efficient generation of UBAC2-deficient cell models for investigation of UBAC2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting UBAC2 exon(s) critical for UBAC2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple UBAC2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UBAC2 CRISPR/Cas9 KO Plasmid (h) and UBAC2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the UBAC2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UBAC2 HDR Plasmid (h) and UBAC2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by UBAC2 homology arms to support homology-directed repair at defined UBAC2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.