UBA2 Antibody (B-6): sc-376305

UBA2 Antibody (B-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects UBA2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). UBA2 (B-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UBA2 protein plays a crucial role in the sumoylation process, which is essential for regulating various cellular functions. Sumoylation, unlike ubiquitination that primarily targets proteins for degradation, modifies proteins to influence stability, localization, and activity, thereby impacting processes such as nuclear transport and transcriptional regulation. UBA2, along with homolog AOS1, forms a heterodimer that activates SUMO-1, facilitating conjugation to target proteins, including key regulators like p53 and MDM2. This interaction maintains cellular homeostasis and responds to stress, making UBA2 a significant player in cellular signaling pathways.

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UBA2 Antibody (B-6) References:

1. Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex.  |  Gong, L., et al. 1999. FEBS Lett. 448: 185-9. PMID: 10217437
2. Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3.  |  Saitoh, H. and Hinchey, J. 2000. J Biol Chem. 275: 6252-8. PMID: 10692421
3. SUMO conjugation and deconjugation.  |  Schwienhorst, I., et al. 2000. Mol Gen Genet. 263: 771-86. PMID: 10905345
4. Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9.  |  Tatham, MH., et al. 2001. J Biol Chem. 276: 35368-74. PMID: 11451954
5. SUMO-1 modification of human cytomegalovirus IE1/IE72.  |  Spengler, ML., et al. 2002. J Virol. 76: 2990-6. PMID: 11861864
6. Versatile protein tag, SUMO: its enzymology and biological function.  |  Kim, KI., et al. 2002. J Cell Physiol. 191: 257-68. PMID: 12012321
7. Molecular features of human ubiquitin-like SUMO genes and their encoded proteins.  |  Su, HL. and Li, SS. 2002. Gene. 296: 65-73. PMID: 12383504
8. SUMO-1 modification of the acute promyelocytic leukaemia protein PML: implications for nuclear localisation.  |  Duprez, E., et al. 1999. J Cell Sci. 112 (Pt 3): 381-93. PMID: 9885291
9. In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2.  |  Okuma, T., et al. 1999. Biochem Biophys Res Commun. 254: 693-8. PMID: 9920803

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-UBA2 Antibody (B-6) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-UBA2 Antibody (B-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

UBA2 Antibody (B-6) Specifications

Alternate Product Name: UBA2

Product Clone Name: B-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 138-292

Epitope Target: UBA2

Epitope Species: human

Product Reactivity/Detection: UBA2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for UBA2 Antibody (B-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin