Ub+1 Antibody (40B3): sc-58450
- Ub+1 Antibody (40B3) is a mouse monoclonal IgG1 Ub+1 antibody provided at 100 µl
- raised against a synthetic peptide of Ub+1 of human origin
- recommended for detection of Ub+1 (the mutant form of ubiquitin produced by molecular frameshift) of human origin by WB and IP; non cross-reactive with Ub
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Ub+1 Antibody (40B3). This work is in progress.
Ub+1 Antibody (40B3) is a mouse monoclonal IgG1 antibody designed to detect Ub+1, a mutant form of ubiquitin resulting from a molecular frameshift, in human samples through western blotting (WB) and immunoprecipitation (IP). Anti-Ub+1 antibody (40B3) does not cross-react with native ubiquitin, ensuring detection of Ub+1. Ubiquitin is a highly conserved 76-amino-acid protein that tags abnormal or misfolded proteins for degradation via the 26S proteasome, maintaining cellular protein homeostasis. The Ub+1 variant arises from a frameshift mutation in the ubiquitin B gene (UBB), leading to a C-terminal extension that disrupts normal ubiquitin function. This alteration impairs the formation of polyubiquitin chains, particularly those linked through Lysine 48, which are crucial for proteasomal recognition and degradation. Consequently, Ub+1 accumulates and has been implicated in neurodegenerative diseases such as Alzheimer′s and Huntington′s, where Ub+1 may interfere with normal protein degradation pathways. Anti-Ub+1 antibody (40B3) serves as a valuable tool for researchers investigating the role of this aberrant protein in disease pathology and the broader mechanisms of protein quality control.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ub+1 Antibody (40B3) | sc-58450 | 100 µl | $322.00 |