U2AF65 Antibody (MC3): sc-53942

U2AF65 Antibody (MC3) is a mouse monoclonal IgG2b kappa light chain antibody that detects U2AF65 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-U2AF65 antibody (MC3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. U2AF65 plays a critical role in the splicing of pre-mRNA, functioning as part of the U2 auxiliary factor complex, which is essential for spliceosome assembly. U2AF65 is located in the nucleus, where U2AF65 interacts with pre-mRNA and other spliceosomal components to facilitate the recognition of branch point sequences during the splicing process. Proper localization of U2AF65 ensures efficient and accurate splicing, which is crucial for the generation of mature mRNA and regulation of gene expression. U2AF65 forms a heterodimer with U2AF35, and together these proteins are indispensable for the recruitment of the U2 small nuclear ribonucleoprotein (snRNP) to the splice site, highlighting U2AF65′s importance in the intricate process of mRNA maturation.

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U2AF65 Antibody (MC3) References:

1. Combined biochemical and electron microscopic analyses reveal the architecture of the mammalian U2 snRNP.  |  Krämer, A., et al. 1999. J Cell Biol. 145: 1355-68. PMID: 10385517
2. Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155.  |  Das, BK., et al. 1999. Mol Cell Biol. 19: 6796-802. PMID: 10490618
3. Identification, purification, and biochemical characterization of U2 small nuclear ribonucleoprotein auxiliary factor.  |  Zamore, PD. and Green, MR. 1989. Proc Natl Acad Sci U S A. 86: 9243-7. PMID: 2531895
4. UHM-ULM interactions in the RBM39-U2AF65 splicing-factor complex.  |  Stepanyuk, GA., et al. 2016. Acta Crystallogr D Struct Biol. 72: 497-511. PMID: 27050129
5. U2AF65 enhances milk synthesis and growth of bovine mammary epithelial cells by positively regulating the mTOR-SREBP-1c signalling pathway.  |  Yu, Y., et al. 2019. Cell Biochem Funct. 37: 93-101. PMID: 30773658
6. U2AF65-Dependent SF3B1 Function in SMN Alternative Splicing.  |  Choi, N., et al. 2020. Cells. 9: PMID: 33317029
7. The U2AF65/circNCAPG/RREB1 feedback loop promotes malignant phenotypes of glioma stem cells through activating the TGF-β pathway.  |  Li, H., et al. 2023. Cell Death Dis. 14: 23. PMID: 36635261
8. The conserved pre-mRNA splicing factor U2AF from Drosophila: requirement for viability.  |  Kanaar, R., et al. 1993. Science. 262: 569-73. PMID: 7692602
9. The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site.  |  Champion-Arnaud, P. and Reed, R. 1994. Genes Dev. 8: 1974-83. PMID: 7958871
10. U2AF homolog required for splicing in vivo.  |  Potashkin, J., et al. 1993. Science. 262: 573-5. PMID: 8211184
11. CUS1, a suppressor of cold-sensitive U2 snRNA mutations, is a novel yeast splicing factor homologous to human SAP 145.  |  Wells, SE., et al. 1996. Genes Dev. 10: 220-32. PMID: 8566755
12. Conservation of structure and subunit interactions in yeast homologues of splicing factor 3b (SF3b) subunits.  |  Igel, H., et al. 1998. RNA. 4: 1-10. PMID: 9436903

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-U2AF65 Antibody (MC3) has 31 citations in a variety of scientific publications.

Primary antibodies like Anti-U2AF65 Antibody (MC3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

U2AF65 Antibody (MC3) Specifications

Alternate Product Name: U2AF2

Product Clone Name: MC3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: U2AF65

Epitope Species: human

Product Reactivity/Detection: U2AF65

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: Xenopus

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 31 citations in various scientific publications

Available Conjugates/Forms for U2AF65 Antibody (MC3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin