U2AF65 Antibody (C-8): sc-166695
- U2AF65 Antibody (C-8) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of U2AF65 of human origin
- recommended for detection of U2AF65 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine and bovine
- m-IgG1 BP-HRP is the preferred secondary detection reagent for U2AF65 Antibody (C-8) for WB applications. This reagent is now offered in a bundle with U2AF65 Antibody (C-8) (see ordering information below).
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U2AF65 Antibody (C-8) is a mouse monoclonal IgG1 antibody that detects U2AF65 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). U2AF65 is a critical component of the U2 auxiliary factor (U2AF) complex, which plays a vital role in the splicing of pre-mRNA by recognizing the poly-pyrimidine tract at the 3′ splice site. This interaction is essential for the recruitment of the U2 small nuclear ribonucleoprotein (snRNP) to the branch point sequence, thereby facilitating the assembly of the spliceosome. U2AF65 extends beyond structural functions to regulate alternative splicing, which can significantly impact gene expression and protein diversity. Dysregulation of U2AF65 has been implicated in various diseases, including cancer, highlighting U2AF65′s potential as a therapeutic target. Anti-U2AF65 antibody (C-8) provides a reliable tool for researchers studying the mechanisms of mRNA splicing and its implications in cellular processes and disease states.
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U2AF65 Antibody (C-8) References:
- Multiple U2AF65 binding sites within SF3b155: thermodynamic and spectroscopic characterization of protein-protein interactions among pre-mRNA splicing factors. | Thickman, KR., et al. 2006. J Mol Biol. 356: 664-83. PMID: 16376933
- The protein factors MBNL1 and U2AF65 bind alternative RNA structures to regulate splicing. | Warf, MB., et al. 2009. Proc Natl Acad Sci U S A. 106: 9203-8. PMID: 19470458
- Perturbation of U2AF65/NXF1-mediated RNA nuclear export enhances RNA toxicity in polyQ diseases. | Tsoi, H., et al. 2011. Hum Mol Genet. 20: 3787-97. PMID: 21725067
- Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. | Wang, W., et al. 2013. Structure. 21: 197-208. PMID: 23273425
- The splicing factor U2AF65 stabilizes TRF1 protein by inhibiting its ubiquitin-dependent proteolysis. | Kim, J. and Chung, IK. 2014. Biochem Biophys Res Commun. 443: 1124-30. PMID: 24389012
- Structure-guided U2AF65 variant improves recognition and splicing of a defective pre-mRNA. | Agrawal, AA., et al. 2014. Proc Natl Acad Sci U S A. 111: 17420-5. PMID: 25422459
- Splicing inhibition of U2AF65 leads to alternative exon skipping. | Cho, S., et al. 2015. Proc Natl Acad Sci U S A. 112: 9926-31. PMID: 26216990
- JMJD6 and U2AF65 co-regulate alternative splicing in both JMJD6 enzymatic activity dependent and independent manner. | Yi, J., et al. 2017. Nucleic Acids Res. 45: 3503-3518. PMID: 27899633
- Cancer-Associated Substitutions in RNA Recognition Motifs of PUF60 and U2AF65 Reveal Residues Required for Correct Folding and 3' Splice-Site Selection. | Kralovicova, J., et al. 2020. Cancers (Basel). 12: PMID: 32664474
- The role of JMJD6/U2AF65/AR-V7 axis in castration-resistant prostate cancer progression. | Tong, D. 2021. Cancer Cell Int. 21: 45. PMID: 33430885
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
U2AF65 Antibody (C-8) | sc-166695 | 200 µg/ml | $322.00 | |||
U2AF65 Antibody (C-8): m-IgG1 BP-HRP Bundle | sc-542668 | 200 µg Ab; 20 µg BP | $361.00 |